大鼠ATP依赖性DNA连接酶Ⅰ(LIG1)多克隆抗体
产品名称： 大鼠ATP依赖性DNA连接酶Ⅰ(LIG1)多克隆抗体
英文名称： Polyclonal Antibody to ATP Dependent DNA ligase I (LIG1)
产品编号： YBB7269Ra01
产品价格： null
产品产地： 中国/上海
品牌商标： 钰博生物
更新时间： 2023-08-17T10:29:50
使用范围： WB, ICC, IHC-P, IHC-F, ELISA
上海钰博生物科技有限公司
- 联系人 : 陈环环
- 地址 : 上海市沪闵路6088号龙之梦大厦8楼806室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 183****2235
- 传真 : 021-60514606
- 邮箱 : shybio@126.com
大鼠ATP依赖性DNA连接酶Ⅰ(LIG1)多克隆抗体
Polyclonal Antibody to ATP Dependent DNA ligase I (LIG1)
[ PRODUCT INFORMATION ]
Immunogen: LIG1, Rat
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant LIG1 (Lys568~Phe875) expressed in E.coli.
Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSG LVPRGSH MASMTGGQQM GRGSEF - KYY GQRAQIHVLE GGEVKIFSRN QEDNSGKYPD IISRIPKIKH PSVTSFILDT EAVAWDREKK QIQPFQVLTT RKRKEVDASE IQVQVCLYAF DLIYLNGESL ARQPLSRRRQ LLRENFVETE GEFVFATSLD TKDIEQIAEF LEQSVKDSCE GLMVKTLDVD ATYEIAKRSH NWLKLKKDYL EGVGDTLDLV VIGAYLGRGK RPGRYGGFLL AAYDEESEEL AAICKLGTGF SDEELEEHHQ NMQALLLPTP RPYVRIDGAV APNHWLDPSI VWEVKCADLT LSPIYRAARG MVDKEKGISL RFPRF
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against LIG1. It has been selected for its ability to recognize LIG1 in immunohistochemical staining and western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ IMAGES ]
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant LIG1 (Lys568~Phe875) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%
[ STORAGE ]
Store at 4 °C for frequent use. Stored at -20°C to -80 °C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
本产品仅供科研使用.请勿用于医药,不能用于临床治疗诊断使用！以实际收货产品说明书为准，网站说明书仅供参考。