EpiTect ChIP qPCR Array

The Human DNA Damage Signaling Pathway EpiTect ChIP qPCR Array profiles the histone modification status or “histone code” of 84 genes involved in DNA damage signaling pathways. Histone modifications regulate chromatin structure and correlate with the transcriptional activity of associated genes. Daily exposure to environmental agents (such as reactive oxygen species, methylating agents, UV light, and other ionizing radiation) and even normal physiological processes (such as replication and recombination) all damage DNA. DNA damage is recognized at cell cycle checkpoints so that the cell can either arrest cell cycle progression and repair the damage before undergoing mitosis, or proceed to apoptosis if the damage is irreparable. Cells must repair DNA damage to prevent mutations from accumulating and to maintain genome integrity and stability. Dysregulation of this signaling pathway via mutation or potentially via histone modifications may occur during cancer progression. Understanding changes in the histone code of DNA damage signaling genes may therefore help define the molecular and epigenetic mechanisms behind oncogenesis. This array includes genes important for cell cycle arrest, genes important for apoptosis, and genes involved in DNA repair. Using chromatin immunoprecipitation and this real-time PCR array, research studies can easily and reliably analyze the histone modification patterns associated with a focused gene panel involved in DNA damage signaling.

Apoptosis: ABL1, AIFM1 (PDCD8), BRCA1, BRCA2, CIDEA, GADD45A, GADD45G, GML, PPP1R15A, RAD21, TP53, TP73.

Cell Cycle:
Cell Cycle Arrest: CHEK1, CHEK2, DDIT3 (CHOP), GADD45A, GML, GTSE1, HUS1, MAP2K6, MAPK12, PPP1R15A, RAD9A, SESN1, ZAK.
Cell Cycle Checkpoint: ATR, BRCA1, FANCG, NBN (NBS1), RAD1, RBBP8, SMC1A (SMC1L1), TP53.
Other Genes Related to the Cell Cycle: ATM, CHAF1A.

DNA Repair:
Damaged DNA Binding: ANKRD17, BRCA1, BRCA2, DDB1, DMC1, ERCC1, FANCG, FEN1, MPG, MSH2, MSH3, N4BP2, NBN (NBS1), OGG1, PNKP, RAD1, RAD18, RAD51, RAD51L1, REV1 (REV1L), SEMA4A, XPA, XPC, XRCC1, XRCC2, XRCC3.
Base-excision Repair: APEX1, DCLRE1A, MBD4, MPG, MUTYH, NTHL1, OGG1, UNG.
Double-strand Break Repair: CIB1, FEN1, MRE11A, NBN (NBS1), PRKDC, RAD21, RAD50, XRCC6 (G22P1), XRCC6BP1 (KUB3).
Mismatch Repair: ABL1, ANKRD17, EXO1, MLH1, MLH3, MSH2, MSH3, MUTYH, N4BP2, PMS1, PMS2, TP73, TREX1.
Other Genes Related to DNA Repair: ATM, ATRX, BTG2, CCNH, CDK7, CHAF1A, CRY1, ERCC2 (XPD), GTF2H1, IGHMBP2, IP6K3, LIG1, MGMT, MNAT1, PCNA, RPA1, SUMO1.