Overview

Product name
Anti-Glycophorin A antibody [HIR2] (FITC) - Aminoterminal end

See all Glycophorin A products (16) ...


Description
Mouse monoclonal [HIR2] to Glycophorin A - Aminoterminal end (FITC)


Conjugation
FITC


Specificity
ab52452 recognises N-terminal, homologous portion of glycophorins A (GPA) and B (GPB) which are single-pass membrane sialoglycoproteins expressed on red blood cells and erythroid precursor cells.


Tested applications
Flow Cytmore details


Cross reactivity
 
Reacts with
Human
 


Immunogen


synthetic peptide (Human, N-terminal)



Properties

Form
Liquid


Storage instructions
Store at +4°C in the dark. Do not freeze.


Storage buffer
Preservative: 15mM Sodium Azide

Constituents: 0.2% BSA, PBS


Concentration

 


    
100tests at mg/ml



Purity
>95% by SDS-PAGE


Purification notes
Prior to conjugation, ab52452 was purified by Protein G affinity chromatography. After conjugation, it was purified by site exclusion chromatography.


Primary antibody notes
ab52452 agglutinates untreated RBCs but it failes to agglutinate papain-treated cells, binds to GPA, but weakly to GPB. The antibody is useful in erythroid cell development studies, because HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).


Clonality
Monoclonal


Clone number
HIR2


Isotype
IgG2b

http://www.abcam.com/Glycophorin-A-antibody-HIR2-FITC-Aminoterminal-end-ab52452.html