NAD/NADH Assay Kit-试剂盒-抗体-生物在线
NAD/NADH Assay Kit

NAD/NADH Assay Kit

商家询价

产品名称: NAD/NADH Assay Kit

英文名称: NAD/NADH Assay Kit

产品编号: ab65348

产品价格: null

产品产地: 英国

品牌商标: abcam

更新时间: null

使用范围:

上海基星生物科技有限公司
  • 联系人 :
  • 地址 : 上海市宝山区友谊路1506号东智广场1109室
  • 邮编 : 201900
  • 所在区域 : 上海
  • 电话 : 138****5983
  • 传真 : 021-50801884
  • 邮箱 : 342786402@qq.com

Overview

Product name

NAD/NADH Assay Kit

Assay type

Quantitative

Product overview

Assay of nicotinamide nucleotides is of continual interest in the studies of energy transforming and redox state of cells or tissues. The NAD/NADH Assay Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio. The NAD Cycling Enzyme Mix in the kit specifically recognizes NADH/NAD in an enzyme cycling reaction. There is no requirement to purify NADH/NAD from samples. The reaction specifically detects NADH and NAD, but not NADP nor NADPH. The enzyme cycling reaction significantly increases the detection sensitivity and specificity. NADt (NAD and NADH) or NADH can be easily quantified by comparing with standard NADH.

Notes

NADH is a sensitive molecule and can easily be used by different enzymes so it is recommended not to leave the lysates longer. The Cell/ tissue extract should also be kept cold. To avoid interference from other enzymes we recommend deproteinate the samples immediately upon harvesting using 10kD spin column (ab93349).

Tested applications

Functional Studiesmore details

Properties

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C. Store in the dark. Avoid repeated freeze / thaw cycles.

Components Identifier 100 tests
NAD Cycling Buffer NM 1 x 15ml
NAD Cycling Enzyme Mix Green 1 vial
NADH Developer Purple 1 vial
NADH Standard (MW:763) Yellow 1 x 152.6µg
NADH/NAD Extraction Buffer NM 1 x 50ml
Stop Solution Red 1 x 1.2ml

http://www.abcam.com/NAD-NADH-Assay-Kit-ab65348.html