Size  100ug
Cat code  200254
Species Cross-reactivity  H
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  34kDa
Sensitivity  This antibody detects endogenous levels of PSME3 and does not cross-react with related proteins.
Immunogen  Purified recombinant human PSME3 protein fragments expressed in E.coli.
UniProt Number  P61289
GeneBank ID  NM_005789
Subcellular location  Nucleus. Cytoplasm By similarity.
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  PA28gamma,Ki antigen,Ki nuclear autoantigen,proteasome activator 28-gamma,11S regulator complex gamma subunit 11S regulator complex subunit gamma,proteasome activator 28 subunit gamma activator of multicatalytic protease subunit 3;PSME3
Gene_symbol  Ki; PA28G; REG-GAMMA; PA28-gamma
Summary  The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Two transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniPort summary  Function  Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a donut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation.
Application Images :  
Western blot detection of PSME3  in HepG2 ,Jurkat&K562 whole cell lysate(1:1000 diluted).
Predicted band size: 30KDa
Observed band size: 34Kda