Cat code 200618-6D1
Species Cross-reactivity H
Key Application WB,IP,ICC
Antibody type Monoclonal antibody
Purified method Affinity purified
Molecular Weight 450kDa
Sensitivity This antibody detects endogenous levels of DNA-PKcs,and does not cross-react with related proteins.
Immunogen Purified recombinant human DNA-PKcs protein fragments expressed in E.coli
UniProt Number P78527
GeneBank ID NM_006904
Subcellular location Nucleus.
Formulation Purified mouse monoclonal in PBS(pH 7.4)containing with 0.2% sodium azide,0.1%BSA & 50% glycerol.
Altername DNA dependent protein kinase catalytic subuni;DNA PKcs;DNA-dependent protein kinase catalytic subunit;DNA-PK catalytic subunit;DNA-PKcs;DNAPK;DNPK1;hyper radiosensitivity of murine scid mutation, complementing 1;HYRC;HYRC1;p350;p460;PRKDC;PRKDC_HUMAN;protein kinase, DNA activated, catalytic polypeptide;XRCC7;
Summary This gene encodes the catalytic subunit of the DNA-dependent protein kinase(DNA-PK).It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination.The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq,Jul 2010]
UniPort summary Function Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage.Involved in DNA nonhomologous end joining(NHEJ)required for double-strand break(DSB)repair and V(D)J recombination.Must be bound to DNA to express its catalytic properties.Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis(DCLRE1C).The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step.Required to protect and align broken ends of DNA.May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage.Found at the ends of chromosomes,suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion.Also involved in modulation of transcription.Recognizes the substrate consensus sequence[ST]-Q.Phosphorylates´Ser-139´ of histone variant H2AX/H2AFX,thereby regulating DNA damage response mechanism.Phosphorylates DCLRE1C,c-Abl/ABL1,histone H1,HSPCA,c-jun/JUN,p53/TP53, PARP1, POU2F1,DHX9,SRF,XRCC1,XRCC1,XRCC4,XRCC5,XRCC6,WRN,MYC and RFA2.Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA.Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.
Application Images :
Western blot detection of PRKDC in Hela & K562 cell lysates using PRKDC antibody(1:1000 diluted).Predicted band size:450KDa,Observed band size:450KDa.
Immunocytochemistry stain of Hela using PRKDC antibody (1:100).
Immunoprecipitation analysis of Hela cell lysate using PRKDC antibody.