CAMK2A + CAMK2B + CAMK2D,钙/钙调素依赖蛋白激酶2b/2γ抗体

产品编号HZ-0541R
英文名称CAMK2A + CAMK2B + CAMK2D
中文名称钙/钙调素依赖蛋白激酶2b/2γ抗体
别 名CaMK2 beta+gamma+delta; CaMK2 beta; CaMK2 gamma; CaMK2 delta; CAMK2G; Calcium/calmodulin dependent protein kinase (CaM kinase) II beta; Calcium/calmodulin dependent protein kinase II beta; Calcium/calmodulin dependent protein kinase IIB; Calcium/calmodulin dependent protein kinase type II beta chain; CAM 2; CaM kinase II beta chain; CaM kinase II beta subunit; CAM2; CAMK 2; CAMK 2B; CaMK II beta subunit; CaMK II subunit beta; CAMK2; CaMK2 beta; CAMK2B; CAMKB; CaMKII beta subunit; CaMKIIB; MGC29528; Proline rich calmodulin dependent protein kinase; CaM kinase II; Calcium/calmodulin-dependent protein; CaM kinase II gamma subunit; CaM-kinase II gamma chain; CaM kinase II delta subunit; CaM-kinase II delta chain; CAM2; CAMK; CAMK-II; CAMK2D; CAMK2G; CAMK2N2; CAMKD; CAMKG; KCC2B; kinase CaMK2-beta.
说 明 书0.1ml 0.2ml
研究领域肿瘤 神经生物学 信号转导 细胞周期蛋白 激酶和磷酸酶 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat,
CAMK2A + CAMK2B + CAMK2D,钙/钙调素依赖蛋白激酶2b/2γ抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量53-73kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CaMK2 beta gamma delta
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CAMK2A + CAMK2B + CAMK2D,钙/钙调素依赖蛋白激酶2b/2γ抗体PubMedPubMed
产品介绍background:
The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Eight transcript variants encoding eight distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008].

Function:
Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplamic reticulum Ca(2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplamic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.

Subunit:
CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other. Interacts with SYNGAP1 and CAMK2N2 (By similarity). Interacts with MPDZ.

CAMK2A + CAMK2B + CAMK2D,钙/钙调素依赖蛋白激酶2b/2γ抗体Subcellular Location:
Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note=In slow-twitch muscle, evenly distributed between longitudinal SR and junctional SR.

Tissue Specificity:
Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle.

Post-translational modifications:
Autophosphorylation of Thr-287 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-287 locks the kinase into an activated state.

Similarity:
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.

Gene ID:
815
816
817;

Database links:
Entrez Gene: 815 Human
Entrez Gene: 816 Human
Entrez Gene: 817 Human
Entrez Gene: 108058 Mouse
Entrez Gene: 12322 Mouse
Entrez Gene: 12323 Mouse
Entrez Gene: 24245 Rat
Entrez Gene: 24246 Rat
Entrez Gene: 25400 Rat
Omim: 114078 Human
Omim: 607707 Human
Omim: 607708 Human
SwissProt: Q13554 Human
SwissProt: Q13557 Human
SwissProt: Q9UQM7 Human
SwissProt: P11798 Mouse
SwissProt: P28652 Mouse
SwissProt: Q6PHZ2 Mouse
SwissProt: P08413 Rat
SwissProt: P11275 Rat
SwissProt: P15791 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

CaMK2b也是丝/苏氨基酸激酶的一种，同属于Ca++/钙调素依赖性蛋白质激酶家族成员。钙／钙调素依赖性蛋白激酶Ⅱb（CaMKⅡb）广泛分布于中枢神经系统内，参与调节神经递质的合成释放、信号转导和突触可塑性的形成以及磷酸化蛋白