CAMKK2,钙调蛋白激酶激酶β抗体

产品编号HZ-6253R
英文名称CAMKK2
中文名称钙调蛋白激酶激酶β抗体
别 名Calcium/calmodulin dependent protein kinase beta; Calcium/calmodulin dependent protein kinase kinase 2; Calcium/calmodulin dependent protein kinase kinase beta; CaM kinase kinase beta; CaM KK beta; CAMKK 2; CAMKK; CaMKK beta; CAMKK beta protein; CAMKKB.
说 明 书0.1ml 0.2ml
研究领域肿瘤 信号转导 转录调节因子 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Pig, Cow, Horse,
CAMKK2,钙调蛋白激酶激酶β抗体产品应用WB=1:100-500 IHC-P=1:100-500 IHC-F=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量55kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CAMKK2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CAMKK2,钙调蛋白激酶激酶β抗体PubMedPubMed
产品介绍background:
The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Seven transcript variants encoding six distinct isoforms have been identified for this gene. Additional splice variants have been described but their full-length nature has not been determined. The identified isoforms exhibit a distinct ability to undergo autophosphorylation and to phosphorylate the downstream kinases. [provided by RefSeq, Jul 2008].

Function:
Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Efficiently phosphorylates 5'-AMP-activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca(2+) signals (By similarity). Seems to be involved in hippocampal activation of CREB1 (By similarity). May play a role in neurite growth. Isoform 3 may promote neurite elongation, while isoform 1 may promoter neurite branching.

Subunit:
Interacts with calmodulin (By similarity).

Subcellular Location:
Nucleus (By similarity). Cytoplasm. Cell projection. Note=Predominantly nuclear in unstimulated cells (By similarity). Found in the cytoplasm and neurites after forskolin induction.

Tissue Specificity:
Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.

Post-translational modifications:
Autophosphorylated and phosphorylated by PKA. Each isoform may show a different pattern of phosphorylation.

CAMKK2,钙调蛋白激酶激酶β抗体Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain.

Database links:
Entrez Gene: 101147428 Gorilla
Entrez Gene: 10645 Human
Entrez Gene: 83506 Rat
Entrez Gene: 699685 Rhesus monkey
Omim: 615002 Human
SwissProt: Q96RR4 Human
SwissProt: O88831 Rat
Unigene: 297343 Human
Unigene: 88589 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: Human prostate tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-CAMKK2 Polyclonal Antibody, Unconjugated(HZ-6253R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining