ICAM1,细胞间粘附分子1抗体

产品编号HZ-6326R
英文名称ICAM1
中文名称细胞间粘附分子1抗体
别 名BB2; CD54 antigen; CD54; ICAM1; ICAM 1; ICAM-1; Cell surface glycoprotein P3.58; Human rhinovirus receptor; Intercellular adhesion molecule 1; Major group rhinovirus receptor; MALA2; MyD10; P3.58; Surface antigen of activated B cells; ICAM1_HUMAN; MALA-2; MyD10; intercellular adhesion molecule 1 precursor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; major group rhinovirus receptor.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 免疫学 信号转导 干细胞 细胞粘附分子
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Dog, Pig, Cow, Rabbit, Sheep,
ICAM1,细胞间粘附分子1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量55kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from pig CD54/ICAM-1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
ICAM1,细胞间粘附分子1抗体PubMedPubMed
产品介绍background:
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008].

Function:
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

Subunit:
Homodimer (Probable). Interacts with human herpesvirus 8 MIR2 protein (Probable). Interacts with MUC1 and promotes cell aggregation in epithelial cells. Interacts with ARHGEF26/SGEF. Binds to coxsackievirus A21 capsid proteins and acts as a receptor for this virus.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Post-translational modifications:
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

Similarity:
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

Gene ID:
3383

ICAM1,细胞间粘附分子1抗体Database links:
Entrez Gene: 3383 Human
Entrez Gene: 15894 Mouse
Entrez Gene: 25464 Rat
Omim: 147840 Human
Unigene: 643447 Human
SwissProt: P05362 Human
SwissProt: P13597 Mouse
SwissProt: Q00238 Rat
Unigene: 435508 Mouse
Unigene: 12 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-ICAM-1 Polyclonal Antibody, Unconjugated(HZ-6326R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining