别 名H2A histone family member Z; H2A.z; H2A/z; H2AFZ; H2AZ; MGC117173; H2AZ_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 表观遗传学
交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Rabbit,
Histone H2A.Z,组蛋白H2AZ抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量13kDa
性 状Lyophilized or Liquid
免 疫 原KLH conjugated synthetic peptide derived from human Histone H2A.Z
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008].
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms an heterodimer with H2B. H2AFZ interacts with INCENP.
Histone H2A.Z,组蛋白H2AZ抗体Post-translational modifications:
Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis (By similarity).
Not phosphorylated (By similarity).
Belongs to the histone H2A family.
Entrez Gene: 3015 Human
Entrez Gene: 51788 Mouse
Entrez Gene: 58940 Rat
Omim: 142763 Human
SwissProt: P04908 Human
SwissProt: P0C0S5 Human
SwissProt: P0C0S8 Human
SwissProt: P20671 Human
SwissProt: Q16777 Human
SwissProt: Q6FI13 Human
SwissProt: Q7L7L0 Human
SwissProt: Q8IUE6 Human
SwissProt: Q93077 Human
SwissProt: Q96KK5 Human
SwissProt: Q96QV6 Human
SwissProt: Q99878 Human
SwissProt: P0C0S6 Mouse
SwissProt: P0C0S7 Rat
Unigene: 119192 Human
Unigene: 117541 Mouse
Unigene: 112573 Rat
Histone H2A.Z,组蛋白H2AZ抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.