GM130,高尔基体自身蛋白GM130抗体上海沪震实业有限公司

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产品名称: GM130,高尔基体自身蛋白GM130抗体
英文名称: Anti-GM130 antibody
抗体货号: HZ-8155R
产品规格: 0.1ml/0.2ml支
级    别: , , , , 分析纯, affinit
产品产地: 中国/美国
品牌商标: HZbscience
价    格: 询价
抗原: 多肽合成
抗原来源: Rabbit
抗体来源: 见说明书
适用物种: Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep
见说明书conjugate:见说明书
Isotype:IgG
应用范围: WB,ELISA,IHC-P,IHC-F,IF
更新时间: 2016-10-19 16:12:00
详细资料:  实验方法技术资料
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诚信指数:305点
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使用范围:仅限科研使用,不能应用于临床
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产品详细描述

GM130,高尔基体自身蛋白GM130抗体

产品编号HZ-8155R
英文名称GM130
中文名称高尔基体自身蛋白GM130抗体
别 名130 kDa cis Golgi matrix protein; 130 kDa cis-Golgi matrix protein; Cis golgi matrix protein GM130; GM130; GM130 autoantigen; GOGA2_HUMAN; GOLGA 2; GOLGA2; Golgi autoantigen; Golgi autoantigen golgin subfamily a 2; Golgi matrix protein GM130; Golgin 95; golgin A2; Golgin subfamily a 2; Golgin subfamily A member 2; Golgin-95; MGC20672; SY11 protein.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 细胞类型标志物
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow,
GM130,高尔基体自身蛋白GM130抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量113kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human GM130
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
GM130,高尔基体自身蛋白GM130抗体PubMedPubMed
产品介绍background:
The docking of transport vesicles to their target membrane is mediated by p115. GM130, a cis-Golgi matrix protein, interacts specifically with p115 and provides a membrane docking site. Both GM130 and p115 are involved in vesicle tethering to Golgi membranes. The amino-terminus of GM130 binds to p115, whereas the carboxy-terminus binds to Golgi membranes. Both Giantin and GM130 compete for binding to p115. Thus, p115-Giantin and p115-GM130 interactions might mediate independent membrane tethering events. Transport from the ER to the cis/medial Golgi compartments requires the action of p115, GM130 and Giantin via a sequential rather than a coordinate mechanism. Mitotic phosphorylation of GM130 at Serine 25 is mediated by Cdc2, prevents binding to p115 and is directly involved in mitotic Golgi fragmentation. GM130 is phosphorylated in prophase as the Golgi complex starts to break down, and remains phosphorylated in metaphase and anaphase. In telophase, GM130 is dephosphorylated by PP2A as the Golgi fragments start to reassemble.

Function:
Golgi auto-antigen; probably involved in maintaining cis-Golgi structure.

Subunit:
Part of a larger oligomeric complex. Interacts with p115. Interacts with RAB1B that has been activated by GTP-binding. Interacts with GORASP1/GRASP65 and ZFPL1.

Subcellular Location:
Golgi apparatus, Golgi stack membrane; Peripheral membrane protein.

GM130,高尔基体自身蛋白GM130抗体Similarity:
Belongs to the GOLGA2 family.

Database links:
UniProtKB/Swiss-Prot: Q08379.3

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-GM130 Polyclonal Antibody, Unconjugated(HZ-8155R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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