Anti-TP53(R175H) Mouse Monoclonal Antibody-抗体-抗体-生物在线
Anti-TP53(R175H) Mouse Monoclonal Antibody

Anti-TP53(R175H) Mouse Monoclonal Antibody

商家询价

产品名称： Anti-TP53(R175H) Mouse Monoclonal Antibody

英文名称： Anti-TP53(R175H) Mouse Monoclonal Antibody

产品编号： 26072

产品价格： null

产品产地： 美国

品牌商标： NewEastbio

更新时间： null

使用范围： ELISA, WB, IHC

武汉纽斯特生物技术有限公司
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  • 所在区域 : 湖北
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  • 邮箱 : neweast3@126.com
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TP53 (R175H) mono [26072]
[100 µL]		  

Catalog Number:  26072
Gene Symbol:  TP53(R175H)
Description:    Anti-TP53(R175H) Mouse Monoclonal Antibody
Background:   TP53 belongs to the p53 proteins family. It is ubiquitously expressed, acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Defects in TP53 are a cause of esophageal cancer (ESCR), lung cancer (LNCR), adrenocortical carcinoma (ADCC), choroid plexus papilloma (CPLPA) and so on.
Immunogen:   A synthetic peptide from the internal region of TP53 which includes the mutation of R175H, human origin
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:1000-1:1000
IHC: 1:50-1:100
Concentration:    1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-TP53(R175H) antibody recognizes TP53(R175H) of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles.
 
Western blot:  
Western blot analysis of recombinant TP53(R175H) and wildtype proteins. Purified His-tagged TP53(R175H) protein(Cat.#10207) and wildtype protein(Cat.#10202) were blotted with anti-TP53(R175H) monoclonal antibody(Cat.# 26072).
Immunofluorescence:  
Immunofluorescence of cells expressing TP53 proteins with anti-TP53 (R175H) antibody. HEK293T cells were transfected with pCDNA3-GFP-TP53 (WT) plasmid (left column) or pCDNA3-GFP-TP53 (R175H) plasmid (right column), then fixed and stained with anti- TP53 (R175H) monoclonal antibody (Cat. # 26072).