Anti-BRAF (D594G) Mouse Monoclonal Antibody-抗体-抗体-生物在线
Anti-BRAF (D594G) Mouse Monoclonal Antibody

Anti-BRAF (D594G) Mouse Monoclonal Antibody

商家询价

产品名称： Anti-BRAF (D594G) Mouse Monoclonal Antibody

英文名称： Anti-BRAF (D594G) Mouse Monoclonal Antibody

产品编号： 26200

产品价格： null

产品产地： 美国

品牌商标： NewEastbio

更新时间： null

使用范围： ELISA, WB, IHC

武汉纽斯特生物技术有限公司
  • 联系人 :
  • 地址 : 武汉市东湖新技术开发区光谷生物城B3-3栋2-3楼
  • 邮编 : 430074
  • 所在区域 : 湖北
  • 电话 : 186****7297
  • 传真 : 027-87561022
  • 邮箱 : neweast3@126.com
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BRAF (D594G) [26200]
[100ul]		  

Catalog Number:  26200
Gene Symbol:  BRAF, BRAF1, RAFB1
Description:   Anti-BRAF (D594G) Mouse Monoclonal Antibody
Background:   BRAF belongs to a family of serine-threonine protein kinases. As a part of a signaling pathway known as the the RAS/MAPK pathway, it plays an important part in many cellular processes. The processes include cell proliferation, differentiation and transcriptional regulation. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.
Immunogen:   A synthetic peptide from the internal region of BRAF which includes the mutation of D594G, human origin.
Applications:   ELISA, WB
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes D594G mutant, but not wild-type BRAF of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  

 

Western blot analysis of recombinant BRAF (D594G) and wildtype proteins. Purified His-tagged BRAF (D594G) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-BRAF (D594G) monoclonal antibody (Cat. #26200).