Anti-Active Rab4 Mouse Monoclonal Antibody
产品名称： Anti-Active Rab4 Mouse Monoclonal Antibody
英文名称： Anti-Active Rab4 Mouse Monoclonal Antibody
产品编号： 26921
产品价格： null
产品产地： 美国
品牌商标： NewEastbio
更新时间： null
使用范围： IP, IHC
武汉纽斯特生物技术有限公司
- 联系人 :
- 地址 : 武汉市东湖新技术开发区光谷生物城B3-3栋2-3楼
- 邮编 : 430074
- 所在区域 : 湖北
- 电话 : 186****7297
- 传真 : 027-87561022
- 邮箱 : neweast3@126.com
| Catalog Number: 26921 |
| Gene Symbol: RAB4 |
| Description: Anti-Active Rab4 Mouse Monoclonal Antibody |
| Background: Rab-4 belongs to the small GTPase Rab family. It interacts with RAB11FIP1, RABEP1, ZFYVE20, RUFY1, SGSM1, SGSM2 and SGSM3. It participates in protein transport and in vesicular traffic. |
| Immunogen: Recombinant full length protein of active Rab4A. |
| Applications: IP, IHC |
| Recommended Dilutions: 1 μg for 1~2 mg total cellular proteins for IP 1:50~100 dilution for IHC |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-active Rab4 antibody recognizes active Rab4 from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Immunoprecipitation/Western blot: |
 |
Rab4A activation assay. Purified GST-tagged Rab4A proteins (Cat. #10130) were immunoprecipitated with the anti-active Rab4 monoclonal antibody (Cat. No. 26921) after treated with GDP (lane 1) or GTPγ S(lane 2), and was blotted with anti-Rab4A polyclonal antibody (Cat. No. 21067). Input control is shown in the bottom panel. |
| Publications: |
| 1. VASP promotes TGF-β activation of hepatic stellate cells by regulating Rab11 dependent plasma membrane targeting of TGF-β receptors Hepatology Volume 61, Issue 1, pages 361–374, January 2015 |
| 2. Rab5 Activity Regulates GLUT4 Sorting into Insulin-Responsive and Non-Insulin-Responsive Endosomal Compartments: A Potential Mechanism for Development of Insulin Resistance Endocrinology. 2014 Sep;155(9):3315-28 |