甲基化赖氨酸抗体
产品名称： 甲基化赖氨酸抗体
英文名称： Methylated (ε-N) Lysine Antibody
产品编号： ICP0501
产品价格： null
产品产地： 加拿大
品牌商标： ImmuneChem
更新时间： null
使用范围： ELISA WB IHC
- 联系人 :
- 地址 : 广西南宁市高新区科园大道68号东盟慧谷11栋203号
- 邮编 : 530007
- 所在区域 : 广西
- 电话 : 137****6451
- 传真 :
- 邮箱 : 735565094@qq.com;info@bl-biotech.com
Catalog # Product Description | ICP0501 The affinity purified rabbit polyclonal anti-methylated lysine antibody was developed using a technique unique to ImmuneChem. The methylated lysine antibodies are affinity purified using N-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The antibodies could be utilized for detection, quantization and isolation of proteins with methylated lysine residues. |  Western blot analysis of the methylated histone from melanoma cells with anti-methylated lysine anti-MeK (ICP0501). Histones were isolated with cold 0.1M HCl extraction followed by: A: Blot with 0.5 μg/mL of anti-MeK (ICP0501) in TBSt and blocked with synthetic methylated BSA 100 μg/mL B: Blot with 0.5 μg/mL of anti-MeK (ICP0501) in TBSt | |
Species | Rabbit | ||
Formulation | 250 μg/mL in Tris-phosphate buffer, 50% glycerol | ||
Immunogen | Methylated KLH conjugates | ||
Purification | The antibody was immunoaffinity purified with epsilon N-methyl lysine on agarose. | ||
Specificity | The antibody recognizes proteins or peptides methylated on lysine residues (mono, di-methyllysine). There are no cross reactions with acetylated proteins. The cross reactivity with tri-methyl lysine has not been tested. | ||
Applications | ELISA; WB; IHC | ||
Scientific Description | MeK is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome C, chromosomal proteins, histones and non-histones as well as neural storage body proteins. It has been suggested that methylation of lysine plays an important role in gene silencing. [1-3] | ||
Storage & Stability | Store product at -20°C. Expiration date is one year from date of shipping if stored properly. | ||
Product Specific References | 2. J. Biol. Chem. 2002. 277: 34655-34657.doi:10.1074/jbc.C200433200 4. Biochem & Biophys Res. Com. 2014. 451 (2): 229-234. doi:10.1016/j.bbrc.2014.07.11 6. Proteomics. 2015. 15 (13): 2166–2176.doi:10.1002/pmic.201400521 本公司还提供以下相关优质产品： |