Recombinant Human Interleukin-15
产品名称: Recombinant Human Interleukin-15
英文名称: IL-T
产品编号: IC-101-15
产品价格: null
产品产地: 美国
品牌商标: XY-Bioscience
更新时间: 2023-08-17T09:55:27
使用范围: > 98 % by SDS-PAGE and HPLC analyses.
上海信裕生物科技有限公司
- 联系人 : 徐经理
- 地址 : 上海市闵行莘庄工业区春东路508号A1-2F
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 152****8802
- 传真 : 021-37680378
- 邮箱 : shxysw02@163.com
Recombinant Human Interleukin-15
Synonyms | IL-T |
Species | 人 |
Accession | P40933 |
GeneID | 3600 |
Source | Escherichia coli. |
Molecular Weight | Approximately 12.9 kDa, a single non-glycosylated polypeptide chain containing 114 amino acids. |
Quantity | 5µg/100µg/1mg |
AA Sequence | NWVNVISDLK KIEDLIQSMH IDATLYTESD VHPSCKVTAM KCFLLELQVI SLESGDASIH DTVENLIILA NNSLSSNGNV TESGCKECEE LEEKNIKEFL QSFVHIVQMF INTS |
Purity | > 98 % by SDS-PAGE and HPLC analyses. |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
Endotoxin | Less than 0.01 EU/µg of rHuIL-15 GMP as determined by LAL method. |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Usage | This GMP product can be for research use or further manufacturing use. |
Quality statement | The manufacture and testing of this product is in compliance with ICH Q7a guidelines. |
Reference | 1. Anderson DM, Johnson L, Glaccum MB, et al. 1995. Genomics, 25: 701-6. 2. Krause H, Jandrig B, Wernicke C, et al. 1996. Cytokine, 8: 667-74. 3. Chirifu M, Hayashi C, Nakamura T, et al. 2007. Nat Immunol, 8: 1001-7. 4. Grabstein KH, Eisenman J, Shanebeck K, et al. 1994. Science, 264: 965-8. 5. Giri JG, Ahdieh M, Eisenman J, et al. 1994. EMBO J, 13: 2822-30. 6. Arena A, Merendino RA, Bonina L, et al. 2000. New Microbiol, 23: 105-12. |
Background | Human Interleukin-15 (IL-15) is expressed by the IL15 gene located on the chromosome 4. It shares approximately 97 % and 73 % sequence identity with simian and murine IL-15, respectively. Both human and simian IL-15 are active on murine cells. IL-15 is secreted by mononuclear phagocytes (and some other cells), especially macrophages following infection by virus. It possesses a variety of biological functions, including stimulating and maintaining of cellular immune responses, especially regulating T and natural killer (NK) cell activation and proliferation. In additionally, it shares many biological properties with IL-2, including T, B and NK cell-stimulatory activities. IL-15 signals through a complex composed of IL-2/IL-15 receptor beta chain. Although IL-15 lacks sequence homology with IL-2, it has recently been shown that both the beta and gamma chains of the IL-2 receptor are utilized for IL-15 binding and signaling. In addition, an IL-15 specific binding protein has also been cloned from a mouse T cell clone |