Recombinant Human Interleukin-7
产品名称: Recombinant Human Interleukin-7
英文名称: LP-1, pre-B cell factor
产品编号: IC-101-07
产品价格: null
产品产地: 美国
品牌商标: XY-Bioscience
更新时间: 2023-08-17T09:55:27
使用范围: > 98 % by SDS-PAGE and HPLC analyses.
上海信裕生物科技有限公司
- 联系人 : 徐经理
- 地址 : 上海市闵行莘庄工业区春东路508号A1-2F
- 邮编 : 200612
- 所在区域 : 上海
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- 传真 : 021-37680378
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Recombinant Human Interleukin-7
Synonyms | LP-1, pre-B cell factor |
Species | 人 |
Accession | P13232 |
GeneID | 3574 |
Source | Escherichia coli. |
Molecular Weight | Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids. |
Quantity | 5µg/100µg/1mg |
AA Sequence | DCDIEGKDGK QYESVLMVSI DQLLDSMKEI GSNCLNNEFN FFKRHICDAN KEGMFLFRAA RKLRQFLKMN STGDFDLHLL KVSEGTTILL NCTGQVKGRK PAALGEAQPT KSLEENKSLK EQKKLNDLCF LKRLLQEIKT CWNKILMGTK EH |
Purity | > 98 % by SDS-PAGE and HPLC analyses. |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine 2E8 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
Endotoxin | Less than 0.01 EU/µg of rHuIL-7 GMP as determined by LAL method. |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Usage | This GMP product can be for research use or further manufacturing use. |
Quality statement | The manufacture and testing of this product is in compliance with ICH Q7a guidelines. |
Reference | 1. Goodwin RG, Lupton S, Schmierer A, et al. 1989. Proc Natl Acad Sci U S A. 86:302-6. 2. Sutherland GR, Baker E, Fernandez KE, et al. 1989. Hum Genet. 82:371-2. 3. Lupton SD, Gimpel S, Jerzy R, et al. 1990. J Immunol. 144:3592-601. 4. Noguchi M, Nakamura Y, Russell SM, et al. 1993. Science. 262:1877-80. 5. Fry TJ, Mackall CL. 2002. Blood. 99:3892-904. 6. Fry TJ, Mackall CL. 2002. J Hematother Stem Cell Res. 11:803-7. |
Background | Interleukin-7 (IL-7) is encoded by the IL7 gene and secreted by stromal cells in the red marrow and thymus. It binds to the IL-7 receptor, a heterodimer consisting of IL-7 receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells and also stimulates proliferation of B cells, T cells and NK cells. Murine IL-7 has approximately 65 % amino acid sequence identity with human IL-7 and both proteins exhibit cross-speciesactivity. IL-7 as an immunotherapy agent has been examinedin many human clinical trials for various malignancies and during HIV infection |