Rabbit Anti-phospho-DOK1 (Tyr398) antibody
|别 名||DOK1 (phospho Y398); DOK1 (phospho Tyr398); p-DOK1 (phospho Y398); docking protein 1 (downstream of tyrosine kinase 1); Docking protein 1 (p62(dok)) (Downstream of tyrosine kinase 1) (pp62).; docking protein 1 62kD (downstream of tyrosine kinase 1); Docking protein 1 62kD; Docking protein 1 62kD; Docking protein 1; DOK 1; DOK1; DOK-1; DOK1_HUMAN; Downstream of tyrosine kinase 1; p62(dok); P62DOK; pp62; pp62.|
|研究领域||肿瘤 心血管 免疫学 信号转导 细胞凋亡 G蛋白偶联受体|
|交叉反应||Human, Mouse, Rat, Dog, Pig, Cow, Horse,|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||53kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated Synthesised phosphopeptide derived from human DOK1 around the phosphorylation site of Tyr398:EG(-Y)EL|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
Docking protein 1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. Docking protein 1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. Docking protein 2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
Interacts with ABL1. Interacts with RasGAP and INPP5D/SHIP1. Interacts directly with phosphorylated ITGB3.
Isoform 1: Cytoplasm. Isoform 3: Cytoplasm, perinuclear region.
Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.
Constitutively tyrosine-phosphorylated. Phosphorylated by TEC (By similarity). Phosphorylated by LYN.
Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
Isoform 3 contains a N-acetylmethionine at position 1.
Belongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain.
Entrez Gene: 1796 Human
Entrez Gene: 13448 Mouse
Entrez Gene: 312477 Rat
Omim: 602919 Human
SwissProt: Q99704 Human
SwissProt: P97465 Mouse
SwissProt: Q4QQV2 Rat
Unigene: 103854 Human
Unigene: 156 Mouse
Unigene: 117974 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications