Rabbit Anti-phospho-EIF4EBP1(Ser111) antibody
|别 名||eIF4EBP1 (phospho S111); eIF4EBP1 (phospho Ser111); p-eIF4EBP1 (Ser111); eIF4EBP1; Eukaryotic translation initiation factor 4E binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E BP1; 4EBP1; BP 1; BP1; eIF4E binding protein 1; eIF4E-binding protein 1; Eukaryotic translation initiation factor 4E binding protein 1; MGC4316; PHAS I; PHASI; PHAS-I; PHAS; 4E-BP1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; Phosphorylated heat and acid stable protein regulated by insulin 1; 4EBP1_HUMAN.|
|研究领域||肿瘤 免疫学 信号转导 结合蛋白|
|交叉反应||Human, Mouse, Rat, Dog, Cow, Horse,|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||13kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated Synthesised phosphopeptide derived from human 4EBP1 around the phosphorylation site of Ser111:EE(p-S)QF|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008].
Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Nonphosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) or mTORC1 phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Interacts with RPTOR.
Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1.
Belongs to the eIF4E-binding protein family.
Entrez Gene: 1978 Human
Entrez Gene: 13685 Mouse
Entrez Gene: 116636 Rat
Omim: 602223 Human
SwissProt: Q13541 Human
SwissProt: Q60876 Mouse
SwissProt: Q62622 Rat
Unigene: 411641 Human
Unigene: 6700 Mouse
Unigene: 11161 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
4E-BP1是一种分子量为10-12kDa 的酸性蛋白，与eIF4G 竞争结合。4E-BPs 对 eIF4E 的结合是可逆的，并依赖于 4E-BP 的***酸化状态。非***酸化的4E-BP1 能与 eiF4E 牢固结合，而***酸化的4E-BP1则不能。 而一些蛋白激酶Akt、 TOR、 MAPK、S6激酶和Cdc2是已知的能通过对35、45、69位的苏氨酸残基或64位的丝氨酸残基进行***酸化而使4E-BP1丧失对eIF4E结合能力的激酶。然而，并非所有的***酸化事件都能同样地阻断4EBP1-eIF4E的相互作用