Rabbit Anti-phospho-EIF4B (Ser504) antibody
|别 名||EIF 4B; EIF-4B; Eukaryotic initiation factor 4B; IF4B_HUMAN.|
|研究领域||肿瘤 免疫学 细胞凋亡 转录调节因子|
|交叉反应||Human, Dog, Cow, Horse, Rabbit,|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||69kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated Synthesised phosphopeptide derived from human EIF4B around the phosphorylation site of Ser504:QQ(p-S)PT|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
eIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot).
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Self-associates and interacts with EIF3 p170 subunit.
Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.
Contains 1 RRM (RNA recognition motif) domain.
Entrez Gene: 1975 Human
Entrez Gene: 75705 Mouse
Entrez Gene: 300253 Rat
Omim: 603928 Human
SwissProt: P23588 Human
SwissProt: Q8BGD9 Mouse
Unigene: 648394 Human
Unigene: 702041 Human
Unigene: 290022 Mouse
Unigene: 391795 Mouse
Unigene: 95954 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications