磷酸化载脂蛋白A1抗体
产品名称: 磷酸化载脂蛋白A1抗体
英文名称: phospho-CD95/FAS (Tyr232)
产品编号: hz-5324R
产品价格: null
产品产地: 中国/上海
品牌商标: HZbscience
更新时间: 2023-08-17T10:24:20
使用范围: WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-50
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Rabbit Anti-phospho-CD95/FAS (Tyr232) antibody
产品编号 | hz-5324R |
英文名称 | phospho-CD95/FAS (Tyr232) |
中文名称 | 磷酸化载脂蛋白A1抗体 |
别 名 | CD95 (phospho Y232); CD95 (phospho Tyr232); p-CD95 (Tyr232); FAS (phospho Y232); FAS (phospho Tyr232); p-FAS (Tyr232); ALPS 1A; ALPS1A; APO 1; Apo 1 antigen; APO 1 cell surface antigen; Apo-1 antigen; APO1; Apo1 antigen; APO1 cell surface antigen; Apoptosis antigen 1; Apoptosis mediating surface antigen FAS; Apoptosis-mediating surface antigen FAS; APT 1; APT1; CD 95; CD 95 antigen; CD95; CD95 antigen; Delta Fas; Delta Fas/APO 1/CD95; Delta Fas/APO1/CD95; FAS 1; FAS 827dupA; Fas AMA; FAS; FAS Antigen; FAS1; FASLG receptor; FASTM; TNF receptor superfamily, member 6; TNFRSF 6; TNFRSF6; TNR6_HUMAN; Tumor necrosis factor receptor superfamily member 6. |
产品类型 | 磷酸化抗体 |
研究领域 | 肿瘤 免疫学 细胞凋亡 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, |
产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 34kDa |
细胞定位 | 细胞膜 分泌型蛋白 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human FAS around the phosphorylation site of Tyr232:SK(p-Y)IT |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: FAS is a receptor for TNFSF6/FASL. The adaptor molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Apoptosis or programmed-cell death is a physiological process essential for the normal development and maintenance of homeostasis in many organisms. This “cellular suicide” can be mediated by the Fas antigen (CD95, APO1), a cell-surface glycoprotein, 40-50kDa, that belongs to the nerve growth factor/tumor necrosis factor (TNF) receptor family. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both (By similarity). It is type I membrane protein. Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adaptor proteins Contains 1 death domain. Function: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). Subunit: Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD. Binds RIPK1 and FAIM2. Interacts with BRE and FEM1B. Interacts with FADD. Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2, 3, 4, 5, 6: Secreted. Tissue Specificity: Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. Post-translational modifications: N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. DISEASE: Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly. Similarity: Contains 1 death domain. Contains 3 TNFR-Cys repeats. Gene ID: 355 Database links: Entrez Gene: 355 Human Entrez Gene: 14102 Mouse Entrez Gene: 246097 Rat Omim: 134637 Human SwissProt: P25445 Human SwissProt: P25446 Mouse SwissProt: Q63199 Rat Unigene: 244139 Human Unigene: 1626 Mouse Unigene: 162521 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. FAS的凋亡信号主要是通过与其胞浆区相关的死亡结构域蛋白FADD介导的。FAS与FasL结合后,FADD一方面通过C端的DD结合FAS, 另一方面通过N端的DED与Caspase-8 N端DED结合,通过Caspase-8诱导效应性Caspase 蛋白酶的激活,并最终导致细胞凋亡的发生。FAS主要表达于活化淋巴细胞、单核细胞、中性粒细胞和成纤维细胞等。 Fas又称作APO-1/CD95,属TNF受体家族。Fas基因编码产物为分子量45KD的跨膜蛋白,分布于胸腺细胞,激活的T和B淋巴细胞,巨噬细胞,肝、脾、肺、心、脑、肠、睾丸和卵巢细胞等。 Fas蛋白与Fas配体结合后,会激活caspase,导致靶细胞走向凋亡 |