Rabbit Anti-phospho-ILK-1(Ser259) antibody
|别 名||ILK-1 (phospho S259); ILK-1 (phospho Ser259); p-ILK-1 (Ser259); 59 kDa serine/threonine protein kinase; 59 kDa serine/threonine-protein kinase; ILK-2; ILK_HUMAN; Integrin linked Kinase; Integrin-linked protein kinase; DKFZp686F1765; EC 188.8.131.52; ILK 1; ILK 2; ILK; ILK1; ILK2; Integrin linked Kinase 2; Integrin linked protein kinase; p59; p59ILK.|
|研究领域||肿瘤 免疫学 神经生物学 信号转导 转录调节因子|
|交叉反应||Human, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||50kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated Synthesised phosphopeptide derived from human ILK-1 around the phosphorylation site of Ser259:CQ(p-S)PP|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
The ILK protein is important in different biological pathways such as cell adhesion, anchorage-dependent cell cycle progression, oncogenic transformation, and growth factor signaling. The kinase activity of ILK is low in non-activated cells; its activity is stimulated by cell-ECM interactions and by certain growth factors. 3 Negative regulation of ILK is mediated by two phosphatases: PTEN, a tumor suppressor lipid sphatase, and ILKAP, a PP2C protein phosphatase. In tumor cells that do not express PTEN protein, ILK is constitutively active.
Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
Interacts with cytoplasmic domain of beta 1 subunit of integrin. Could also interacts with beta 2, beta 3 and/or beta 5 subunit of integrin. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with parvins and probably TGFB1I1. Interacts (via ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but independent of the kinase activity of EPHA1.
Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
Autophosphorylated on serine residues.
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 5 ANK repeats.
Contains 1 protein kinase domain.
Entrez Gene: 3611 Human
Entrez Gene: 16202 Mouse
Entrez Gene: 170922 Rat
Omim: 602366 Human
SwissProt: Q13418 Human
SwissProt: O55222 Mouse
SwissProt: Q99J82 Rat
Unigene: 5158 Human
Unigene: 706355 Human
Unigene: 274846 Mouse
Unigene: 95042 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.