Rabbit Anti-phospho-CK18(Ser34) antibody
|别 名||Cytokeratin 18 (phospho S34); p-Cytokeratin 18 (phospho S34); Cell proliferation inducing gene 46 protein; Cell proliferation inducing protein 46; Cell proliferation-inducing gene 46 protein; CK 18; CK-18; CK18; CYK 18; CYK18; Cytokeratin 18; Cytokeratin endo B; Cytokeratin-18; Cytokeratin18; K 18; K18; K1C18_HUMAN; Keratin 18; Keratin D; Keratin type I cytoskeletal 18; keratin, type I cytoskeletal 18; Keratin-18; Keratin18; Kerd; KRT18.|
|研究领域||肿瘤 信号转导 细胞类型标志物|
|交叉反应||Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Guinea Pig,|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||48kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthesised phosphopeptide derived from human CK18 around the phosphorylation site of Ser34 [AA(p-S)VY]:AA(p-S)VY|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].
When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Heterotetramer of two type I and two type II keratins. KRT18 associates with KRT8. Interacts with the thrombin-antithrombin complex (By similarity). Interacts with PNN, HCV core protein and mutated CFTR. Interacts with YWHAE, YWHAH and YWHAZ only when phosphorylated. Interacts with DNAJB6, TCHP and TRADD.
Cytoplasm, perinuclear region. Nucleus, nucleolus.
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-231 by either caspase-3, caspas-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residue.
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
Belongs to the intermediate filament family.
Entrez Gene: 506480 Cow
Entrez Gene: 3875 Human
Entrez Gene: 16668 Mouse
Entrez Gene: 294853 Rat
Omim: 148070 Human
SwissProt: P05783 Human
SwissProt: P05784 Mouse
SwissProt: Q5BJY9 Rat
Unigene: 406013 Human
Unigene: 22479 Mouse
Unigene: 103924 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.