Rabbit Anti-phospho-PKMYT1 (Thr495) antibody
|别 名||PKMYT1(phospho T495);cdc2 inhibitory kinase; Membrane associated tyrosine and threonine specific cdc2 inhibitory kinase; Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; MYT1; Myt1 kinase; PKMYT 1; Pkmyt1; PMYT1_HUMAN; Protein kinase membrane associated tyrosine/threonine 1; Protein kinase Myt1; DKFZp547K1610; FLJ20093.|
|研究领域||免疫学 信号转导 细胞周期蛋白 转录调节因子 激酶和***酸酶|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||55kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated Synthesised phosphopeptide derived from human PKMYT1 around the phosphorylation site of Thr495:ED(p-T)LD|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.
Interacts with CDC2-CCNB1 complex. Can also interact with PIN1 when phosphorylated by CDC2-CCNB1.
Endoplasmic reticulum membrane. Golgi apparatus membrane.
Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
Contains 1 protein kinase domain.
Entrez Gene: 9088 Human
Entrez Gene: 268930 Mouse
Entrez Gene: 287101 Rat
Omim: 602474 Human
SwissProt: Q99640 Human
SwissProt: Q9ESG9 Mouse
Unigene: 77783 Human
Unigene: 182193 Mouse
Unigene: 204349 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.