Rabbit Anti-phospho-VAV1 (Tyr174) antibody
|别 名||VAV1(phospho Y174); Oncogene vav; p95Vav; Protooncogene vav; Proto-oncogene vav; VAV 1; VAV 1 oncogene; VAV; Vav proto oncogene; Vav proto-oncogene; VAV_HUMAN; VAV1.|
|研究领域||肿瘤 免疫学 染色质和核信号 信号转导 转录调节因子 激酶和***酸酶 G蛋白偶联受体 t-淋巴细胞 b-淋巴细胞|
|交叉反应||Human, Mouse, Rat, Dog, Pig, Horse,|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||98kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated Synthesised phosphopeptide derived from human VAV1 around the phosphorylation site of Tyr174:EI(p-Y)ED|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012].
Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
May interact with CCPG1. Interacts with APS, DOCK2, GRB2, GRB3, DOCK2, SLA, TEC and ZNF655/VIK. Interacts with SIAH2; without leading to its degradation. Associates with BLNK, PLCG1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with CBLB; which inhibits tyrosine phosphorylation and down-regulates activity. Interacts with SHB and CLNK. Interacts with THEMIS2. Interacts with HCK. Interacts with NEK3 and this interaction is prolactin-dependent. Interacts with ITK. Interacts with PTK2B/PYK2. Interacts (via SH2 domain) with SYK. Interacts with ANKRD54.
Widely expressed in hematopoietic cells but not in other cell types.
Phosphorylated on tyrosine residues by HCK in response to IFNG and bacterial lipopolysaccharide (LPS). Phosphorylated by FYN.
Contains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
Entrez Gene: 7409 Human
Entrez Gene: 22324 Mouse
Entrez Gene: 25156 Rat
Omim: 164875 Human
SwissProt: P15498 Human
SwissProt: P27870 Mouse
SwissProt: P54100 Rat
Unigene: 116237 Human
Unigene: 248172 Mouse
Unigene: 48861 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.