Rabbit Anti-phospho-PTK9 (Tyr321) antibody
|别 名||PTK9 (phospho-Tyr321); PTK9 (phospho-Y321); p-PTK9 (Tyr321); p-TWF1(Y321); A6; A6 protein tyrosine kinase; Protein A6; Protein tyrosine kinase 9; PTK9; PTK9 protein tyrosine kinase 9; TWF 1; TWF1; Twinfilin 1; Twinfilin actin binding protein, homolog 1; Twinfilin; Twinfilin, actin-binding protein, homolog 1 (Drosophila); Twinfilin, Drosophila, homolog of, 1; TWF1_HUMAN; Twinfilin-1.|
|研究领域||免疫学 信号转导 结合蛋白 细胞骨架|
|交叉反应||Human, Mouse, Rat, Chicken, Dog, Pig, Cow,|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||40kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated Synthesised phosphopeptide derived from human TWF1 around the phosphorylation site of Tyr321:GD(p-Y)LE|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
PTK9 is an actin-binding protein involved in motile and morphological processes. It inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. PTK9 seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.
Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.
Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF2 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down-regulated.
Cytoplasm. Cytoplasm, cytoskeleton. Note=Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts.
Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver.
Phosphorylated on serine and threonine residues.
Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.
Contains 2 ADF-H domains.
Entrez Gene: 5756 Human
Entrez Gene: 506683 Cow
Entrez Gene: 19230 Mouse
Entrez Gene: 315265 Rat
Omim: 610932 Human
SwissProt: Q56JV6 Cow
SwissProt: Q12792 Human
SwissProt: Q91YR1 Mouse
SwissProt: Q5RJR2 Rat
Unigene: 5944 Cow
Unigene: 189075 Human
Unigene: 309867 Mouse
Unigene: 162971 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications