Rabbit Anti-phospho-MLF1 Interacting Protein(Thr78) antibody
|英文名称||phospho-MLF1 Interacting Protein(Thr78)|
|别 名||MLF1 Interacting Protein/PBIP1(phospho T78); MLF1 Interacting Protein/PBIP1 (phospho T78);MLF1 Interacting Protein (phospho T78); MLF1 Interacting Protein (phospho Thr78); p-MLF1 Interacting Protein (Thr78); CENP U; CENP50; CENPU; centromere protein U; HHV8 LNAIP1; ICEN24; Kaposi Sarcoma Herpesvirus latent nuclear antigen interacting protein 1; KLIP-1; KLIP1; KSHV latent nuclear antigen interacting protein 1; KSHV LNAIP1; LNAIP 1; LNAIP-1; MLF1IP1; PBIP1; CENPU_HUMAN.|
|研究领域||肿瘤 免疫学 信号转导 细胞周期蛋白 激酶和***酸酶|
|交叉反应||Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep,|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||48kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthesised phosphopeptide derived from human MLF1 Interacting Protein around the phosphorylation site of Thr78:HS(p-T)AI|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
MLF1 Interacting protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a component of the CENPA nucleosome-associated complex (CENPA NAC), which plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. This protein is involved in transcriptional repression and interacts with the N-terminal domain of Kaposi Sarcoma Herpesvirus latent nuclear antigen (KSHV LNA).
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression.
Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Interacts with the N-terminal domain of Kaposi's sarcoma-associated herpesvirus latent nuclear antigen (LNA). Interacts with MLF1. Interacts with PLK1.
Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Note=Localizes in the kinetochore domain of centromeres. Colocalizes with PLK1 at the interzone between the inner and the outer kinetochore plates.
Expressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells. Expressed at high levels in glioblastoma cell lines.
Phosphorylated by PLK1 at Thr-78, creating a self-tethering site that specifically interacts with the polo-box domain of PLK1.
Entrez Gene: 617866 Cow
Entrez Gene: 79682 Human
Entrez Gene: 71876 Mouse
Entrez Gene: 306464 Rat
Omim: 611511 Human
SwissProt: Q2KIW5 Cow
SwissProt: Q71F23 Human
SwissProt: Q8C4M7 Mouse
SwissProt: Q4V8G7 Rat
Unigene: 575032 Human
Unigene: 217385 Mouse
Unigene: 22108 Mouse
Unigene: 128609 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.