Rabbit Anti-phospho-Proteasome 20S beta 7(Ser214) antibody
|英文名称||phospho-Proteasome 20S beta 7(Ser214)|
|别 名||Proteasome 20S beta 7 (phospho S214); Proteasome 20S beta 7 (phospho Ser214); p-Proteasome 20S beta 7 (S214); p-Proteasome 20S beta 7 (Ser214); 20S Proteasome β7; Macropain chain Z; Multicatalytic endopeptidase complex chain Z; Proteasome (prosome macropain) subunit beta type 7; Proteasome beta 7 subunit; Proteasome catalytic subunit 2; Proteasome subunit alpha; Proteasome subunit beta 7; Proteasome subunit beta type-7; Proteasome subunit Z; PSB7_HUMAN; PSMB7; PUP1.|
|研究领域||肿瘤 细胞生物 细胞周期蛋白 细胞分化|
|交叉反应||Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,|
|产品应用||ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||25kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated Synthesised phosphopeptide derived from human Proteasome 20S beta 7 around the phosphorylation site of Ser214:161-260/277|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity.The proteasome represents a large protein complex that exists inside all eukaryotes and archaea, and in some bacteria. The main function of proteasomes is to degrade unnecessary or damaged proteins by proteolysis. The most common form of the proteasome, known as the 26S Proteasome, contains one 20S Proteasome core particle structure and two 19S regulatory caps. The 20S Proteasome core is hollow and forms an enclosed cavity, where proteins are degraded, as well as openings at the two ends to allow the target protein to enter. The 20S Proteasome core particle contains many subunits, depending on the organism.
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity.
The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic equivalent immune-specific subunit PSMB10. Interacts with HIV-1 TAT protein.
Expressed at a low level in colonic mucosa. Up-regulated in colorectal cancer tissues.
Belongs to the peptidase T1B family.
Entrez Gene: 5695 Human
Entrez Gene: 19177 Mouse
Entrez Gene: 100037992 Pig
Entrez Gene: 85492 Rat
Omim: 604030 Human
SwissProt: Q2TBP0 Cow
SwissProt: Q99436 Human
SwissProt: P70195 Mouse
SwissProt: A1XQU1 Pig
SwissProt: Q9JHW0 Rat
Unigene: 213470 Human
Unigene: 389251 Mouse
Unigene: 3846 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications