Rabbit Anti-phospho-ATP1A1 (Ser 943) antibody
|英文名称||phospho-ATP1A1 (Ser 943)|
|别 名||alpha 1 Sodium Potassium ATPase (phospho S943); alpha 1 Sodium Potassium ATPase; A1A1; AT1A1; AT1A1_HUMAN; Atpa-1; ATPase Na+/K+ transporting alpha 1 polypeptide; ATPase Na+/K+ transporting subunit alpha 1; BC010319; EC 22.214.171.124; MGC3285; MGC38419; MGC51750; Na K ATPase alpha A catalytic polypeptide; Na K ATPase catalytic subunit alpha A protein; Na(+)/K(+) ATPase 1; Na(+)/K(+) ATPase alpha-1 subunit; Na+, K+ ATPase alpha subunit; Na+/K+ ATPase alpha 1 subunit; Na+/K+ ATPase 1; Na,K ATPase alpha 1 subunit; Nkaa1b; Sodium potassium ATPase alpha 1 polypeptide; Sodium pump 1; Sodium pump subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; Sodium/potassium-transporting ATPase subunit alpha-1.|
|研究领域||神经生物学 信号转导 通道蛋白 G蛋白偶联受体 G蛋白信号|
|交叉反应||Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, Sheep,|
|产品应用||ELISA=1:500-1000 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||112kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated Synthesised phosphopeptide derived from human Na,K-ATPase alpha-1 around the phosphorylation site of Ser943:901-1023/1023|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
The ubiquitously expressed sodium/potassium-ATPase (Na+/K+-ATPase) exists as a oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na+ ions and two K+ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na+/K+-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport.The Alpha subunit contains the binding sites for ATP and the cations; the glycosylated Beta subunit ensures correct folding and membrane insertion of the Alpha subunits. The small subunit co-localizes with the Alpha subunit in nephron segments, where it increases the affinity of Na+/K+-ATPase for ATP. The Betasubunit, but not the subunit, is essential for normal activity of Na+/K+-ATPase.
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Interacts with SIK1 (By similarity). Composed of three subunits: alpha (catalytic), beta and gamma. Binds the HLA class II histocompatibility antigen, DR1.
Cell membrane; Multi-pass membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Phosphorylation on Tyr-10 modulates pumping activity. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity (By similarity).
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
Entrez Gene: 476 Human
Entrez Gene: 11928 Mouse
Entrez Gene: 24211 Rat
Omim: 182310 Human
SwissProt: P05023 Human
SwissProt: Q8VDN2 Mouse
SwissProt: P06685 Rat
Unigene: 371889 Human
Unigene: 280103 Mouse
Unigene: 217534 Rat
Unigene: 2992 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications