Rabbit Anti-phospho-Cytokeratin 8 (Ser73) antibody
|英文名称||phospho-Cytokeratin 8 (Ser73)|
|别 名||Cytokeratin 8 (phospho S73); p-Cytokeratin 8 (phospho S73); card2; Cardiac autoantigen 2 120kD; CK 8; CK-8; ck8; Cyk 8; cyk8; CYKER; Cytokeratin-8; Cytokeratin endo A; Cytokeratin-8; Cytokeratin8; DreK8; EndoA; k0; CYK8; k2c8; K2C8_HUMAN; k8; Keratin 8; Keratin type ii cytoskeletal 8; Keratin, type II cytoskeletal 8; Keratin-8; Keratin8; KO; Krt 2.8; Krt 8; krt8; KRT-8; MGC118110; MGC174782; MGC53564; MGC85764; sb:cb186; Type-II keratin Kb8.|
|研究领域||肿瘤 细胞生物 免疫学 信号转导 细胞类型标志物|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||53kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthesised phosphopeptide derived from human CK8 around the phosphorylation site of Ser73:LL(p-S)PL|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012].
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Heterotetramer of two type I and two type II keratins. KRT8 associates with KRT18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP. Interacts with APEX1.
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Defects in KRT8 are a cause of cirrhosis (CIRRH) [MIM:215600]..
Belongs to the intermediate filament family.
Entrez Gene: 3856 Human
Entrez Gene: 16691 Mouse
Entrez Gene: 25626 Rat
Omim: 148060 Human
SwissProt: P05787 Human
SwissProt: P11679 Mouse
SwissProt: Q10758 Rat
Unigene: 533782 Human
Unigene: 708445 Human
Unigene: 358618 Mouse
Unigene: 11083 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.