Rabbit Anti-phospho-alpha Adducin (Thr445) antibody
|英文名称||phospho-alpha Adducin (Thr445)|
|别 名||Adducin (phospho T445); p-Adducin (phospho T445); ADD1; ADDA; Adducin 1 (alpha); Adducin 1; Alpha adducin; ADDA_HUMAN.|
|研究领域||肿瘤 细胞生物 信号转导 细胞骨架|
|交叉反应||Human, Mouse, Rat,|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||80kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthesised phosphopeptide derived from human alpha Adducin around the phosphorylation site of Thr445:EK(p-T)RW|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha and beta adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha and gamma adducins are ubiquitously expressed. In contrast, beta adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca2+/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit.
Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
Belongs to the aldolase class II family. Adducin subfamily.
Entrez Gene: 118 Human
Entrez Gene: 11518 Mouse
Entrez Gene: 24170 Rat
Omim: 102680 Human
SwissProt: P35611 Human
SwissProt: Q9QYC0 Mouse
SwissProt: Q63028 Rat
Unigene: 183706 Human
Unigene: 289106 Mouse
Unigene: 5788 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.