Rabbit Anti-phospho-DAP Kinase 2 (Ser318) antibody
|英文名称||phospho-DAP Kinase 2 (Ser318)|
|别 名||DAP Kinase 2 (phospho S318); p-DAP Kinase 2 (phospho S318); Death associated protein kinase 2; DAK 2; DAK2; DAP kinase related protein 1; DAPK 2; DAPK2; Death associated protein kinase 2; DRP 1; DRP1; DRP-1; MGC119312; DAP kinase 2; DAP kinase related protein 1; DAP kinase related protein 1 beta isoform; DAP-kinase-related protein 1; DAPK 2; DAPK2; DAPK2_HUMAN; Death associated protein kinase 2; Death-associated protein kinase 2; DRP 1; DRP-1; DRP1; MGC119312.|
|研究领域||细胞生物 信号转导 细胞凋亡 激酶和***酸酶|
|交叉反应||Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig,|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||41kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthesised phosphopeptide derived from human DAP Kinase 2 around the phosphorylation site of Ser318:KL(p-S)FS|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
Protein kinase termed (DAPK2) dependant on calcium/calmodulin (Ca2+/CaM) contains an N-terminal protein kinase domain followed by a conserved CaM-binding domain with significant homologies to those of DAP kinase, a protein kinase involved in apoptosis. Overexpression of DAPK2 significantly induced the morphological changes characteristic of apoptosis. Results indicate that DAPK2 is an additional member of DAP kinase family involved in apoptotic signaling.
Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport. Isoform 1 and isoform 4 inhibit peroxisomal division when overexpressed.
Homodimer in its autoinhibited state. Active as monomer. Isoform 2 but not isoform 1 can interact with ATF4.
Cytoplasm. Cytoplasmic vesicle, autophagosome lumen.
Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons.
Autophosphorylation at Ser-318 inhibits its catalytic activity. Dephosphorylated at Ser-318 in response to activated Fas and TNF-alpha receptors.
Belongs to the protein kinase superfamily.
CAMK Ser/Thr protein kinase family.
DAP kinase subfamily.
Contains 1 protein kinase domain.
Entrez Gene: 23604 Human
Entrez Gene: 13143 Mouse
Entrez Gene: 300799 Rat
Omim: 604727 Human
SwissProt: Q9UIK4 Human
SwissProt: Q8VDF3 Mouse
Unigene: 237886 Human
Unigene: 304472 Mouse
Unigene: 335252 Mouse
Unigene: 98325 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic application