Rabbit Anti-phospho-NIFK (Thr238) antibody
|别 名||NIFK (phospho T238); NIFK (phospho Thr238); p-NIFK(T238); p-NIFK(Thr238); hNIFK; MKI67 FHA Domain Interacting Nucleolar Phosphoprotein; MKI67IP; NOPP34; Nucleolar phosphoprotein Nopp34; Nucleolar protein interacting with the FHA domain of pKI 67; pKi67; MK67I_HUMAN.|
|交叉反应||Human, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig,|
|产品应用||ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||34kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthesised phosphopeptide derived from human NIFK/MKI67IP around the phosphorylation site of Thr238:VC(p-T)PT|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq, Jan 2009].
Binds to the FHA domain of MKI67; this interaction is enhanced in mitosis.
Nucleus, nucleolus. Chromosome. Note=Localizes to mitotic chromosomes in conjunction with MKI67.
Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.
Contains 1 RRM (RNA recognition motif) domain.
Entrez Gene: 84365 Human
Entrez Gene: 67949 Mouse
Omim: 611970 Human
SwissProt: Q8TB66 Human
SwissProt: Q9BYG3 Human
SwissProt: Q91VE6 Mouse
Unigene: 367842 Human
Unigene: 280311 Mouse
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic application