Rabbit Anti-phospho-Topoisomerase II alpha (Ser1525) antibody\
|英文名称||phospho-Topoisomerase II alpha (Ser1525)|
|别 名||Topoisomerase II alpha (phospho S1525); alpha isozyme; ATP hydrolyzing DNA topoisomerase II alfa; DNA gyrase; DNA topoisomerase 2 alpha; DNA topoisomerase 2-alpha; DNA topoisomerase II 170 kD; DNA topoisomerase II alpha isozyme; DNA topoisomerase II; DNA Topoisomerase2; TOP 2A; TOP2; TOP2A; TOP2A_HUMAN; Topoisomerase DNA II alpha 170kDa; TP2A; Topo IIα; Topo-IIα.|
|研究领域||细胞生物 发育生物学 表观遗传学|
|交叉反应||Human, Mouse, Rat,|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||174kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthesised phosphopeptide derived from human Topoisomerase II alpha around the phosphorylation site of Ser1525:EE(p-S)D|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
Phosphorylation has no effect on catalytic activity.
Belongs to the type II topoisomerase family.
Entrez Gene: 7153 Human
Entrez Gene: 21973 Mouse
Entrez Gene: 360243 Rat
Omim: 126430 Human
SwissProt: P11388 Human
SwissProt: Q01320 Mouse
SwissProt: P41516 Rat
Unigene: 156346 Human
Unigene: 4237 Mouse
Unigene: 90996 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications