Rabbit Anti-phospho-LIM Kinase 2 (Thr505) antibody
|英文名称||phospho-LIM Kinase 2 (Thr505)|
|别 名||LIM Kinase 1 (phospho T505); p-LIM Kinase 1 (phospho T505); LIM domain kinase 2; LIMK 2; LIMK-2; LIMK2; LIMK2_HUMAN.|
|研究领域||细胞生物 信号转导 激酶和***酸酶|
|交叉反应||Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Zebrafish, Sheep,|
|产品应用||ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||70kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthesised phosphopeptide derived from human LIM Kinase 1 around the phosphorylation site of Thr505:RY(p-T)VV|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
Phosphorylated on serine and/or threonine residues by ROCK1.
Belongs to the protein kinase superfamily.
TKL Ser/Thr protein kinase family.
Contains 2 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain.
Entrez Gene: 3985 Human
Entrez Gene: 16886 Mouse
Entrez Gene: 29524 Rat
Omim: 601988 Human
SwissProt: P53671 Human
SwissProt: O54785 Mouse
SwissProt: P53670 Rat
Unigene: 474596 Human
Unigene: 124176 Mouse
Unigene: 390323 Mouse
Unigene: 442189 Mouse
Unigene: 11013 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applicatio