Rabbit Anti-phospho-MAPK6 (Ser189) antibody
|别 名||MAPK6 (phospho S189); p-MAPK6 (phospho S189); ERK-3; ERK3; ERK 3; ERK-3; Extracellular signal regulated kinase 3; Extracellular signal regulated kinase p97; Extracellular signal-regulated kinase 3; MAP kinase 6; MAP kinase isoform p97; MAPK 6; MAPK6; Mitogen activated protein kinase 6; Mitogen-activated protein kinase 6; MK06_HUMAN; p97 MAPK; p97-MAPK; PRKM6; Protein kinase mitogen activated 5; Protein kinase mitogen activated 6.|
|研究领域||细胞生物 信号转导 激酶和***酸酶|
|交叉反应||Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||83kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthesised phosphopeptide derived from human MAPK6 around the phosphorylation site of Ser189:HL(p-S)EG|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
Mitogen-activated protein kinase (MAPK) signaling pathways involve closely related MAP kinases, including extracellular-signal-related kinase 3 (ERK 3, also designated PRKM6 and p97MAPK). Serum, growth factors and phorbol esters can initiate ERK 3 signaling pathways. Despite lacking a definitive nuclear localization sequence, ERK 3 constitutively localizes to the nucleus upon activation. p38 pathway activation-dependent upregulation of ERK 3 is independent of the status of p53, Bcl-2 and caspase-3 during cell stress and damage induced by proteasome inhibition, suggesting ERK 3 in part mediates intracellular defense or cell rescue. The human ERK 3 gene maps to chromosome 15q21.2 and encodes a 721 amino acid protein. Function : Phosphorylates microtubule-associated protein 2 (MAP2). May promote entry in the cell cycle.
Phosphorylates microtubule-associated protein 2 (MAP2). May promote entry in the cell cycle.
Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.
Dually phosphorylated on Thr-626 and Tyr-628, which activates the enzyme.
Belongs to the protein kinase superfamily.
CMGC Ser/Thr protein kinase family.
MAP kinase subfamily.
Contains 1 protein kinase domain.
Entrez Gene: 5597 Human
Entrez Gene: 50772 Mouse
Entrez Gene: 58840 Rat
Omim: 602904 Human
SwissProt: Q16659 Human
SwissProt: Q61532 Mouse
SwissProt: P27704 Rat
Unigene: 411847 Human
Unigene: 480076 Mouse
Unigene: 88457 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications