Rabbit Anti-phospho-MYL9 (Thr19 + Ser20) antibody
|英文名称||phospho-MYL9 (Thr19 + Ser20)|
|别 名||MYL9 (phospho T19 + S20); p-MLC(Thr19 + Ser20); phospho-MLC(Thr19 + Ser20); p-Myosin light chain(Thr19 + Ser20); MYL9_HUMAN; Myosin regulatory light polypeptide 9; 20 kDa myosin light chain; LC20; MLC-2C; Myosin RLC; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1; MLC2; MRLC1; MYRL2.|
|研究领域||心血管 细胞生物 信号转导 干细胞 细胞骨架 细胞膜蛋白|
|交叉反应||Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Danio|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||20kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthesised phosphopeptide derived from human MYL9 around the phosphorylation site of Thr19 + Ser20:RA(p-T)(p-S)N|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
MYL9 is the myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. MYL9 protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for MYL9 and the deduced 172-amino acid protein is highly conserved, with only 3 differences between the human and chicken proteins. Light chain phosphorylation causes the folded monomeric form of myosin to extend and assemble into filaments. This observation established the involvement of the MYL9 in conformational transitions of smooth muscle myosin.
Myosin light chain (MLC) is a subunit of the conventional myosins (e.g. myosin II). In smooth muscle and non-muscle cells conventional myosins mediate a wide variety of contractile events including cytokinesis, cell motility, and smooth muscle contraction. MLC is phosphorylated by multiple serine-threonine kinases such as Rho-kinase and PAK, however myosin light chain kinase (MLCK) acts as the primary kinase. Contractile activity of conventional myosins is regulated by phosphorylation of MLC on several residues.
Myosin is an hexamer of 2 heavy chains and 4 light chains.
Smooth muscle tissues and in some, but not all, nonmuscle cells.
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge (By similarity).
Contains 3 EF-hand domains.
Entrez Gene: 10398 Human
Entrez Gene: 85366 Human
Entrez Gene: 228785 Mouse
Entrez Gene: 98932 Mouse
Entrez Gene: 117558 Rat
Entrez Gene: 296313 Rat
Omim: 606566 Human
Omim: 609905 Human
SwissProt: P20689 Human
SwissProt: P24844 Human
SwissProt: Q8VCR8 Mouse
SwissProt: Q9CQ19 Mouse
SwissProt: P20689 Rat
SwissProt: Q64122 Rat
Unigene: 504687 Human
Unigene: 271770 Mouse
Unigene: 228729 Rat
Unigene: 6870 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications