磷酸化丝裂原活化蛋白激酶1单克隆抗体上海沪震实业有限公司

性能参数

产品名称: 磷酸化丝裂原活化蛋白激酶1单克隆抗体
英文名称: phospho-Erk 1/2 (Tyr222 + Tyr205)
抗体货号: hz-33353M
产品规格: 100ul
级    别: , , 分析纯,
产品产地: 中国/上海
品牌商标: HZbscience
价    格: 1580元
抗原: IgG
抗原来源: Rabbit
抗体来源: Rabbit
适用物种: 见说明书
应用范围: IHC-P=1:200-500 IHC-F=1:200-500 ICC=1:100-500 IF=1:200-500
更新时间: 2018/4/18 14:33:00
详细资料:  实验方法技术资料
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使用范围:仅限科研使用,不能应用于临床
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产品详细描述

 Mouse Anti-phospho-Erk 1/2 (Tyr222 + Tyr205)  antibody 

 

产品编号 hz-33353M
英文名称 phospho-Erk 1/2 (Tyr222 + Tyr205)
中文名称 ***酸化丝裂原活化蛋白激酶1单克隆抗体
别    名 Phospho-ERK 1/2 (Y222/205); Erk1 (pY222/pY205); ERK/MAPK(phospho Y222/Y205); ERK1 (phospho Y222); p-ERK1 (phospho Y222); p44/42 MAP Kinase(Phospho-Tyr205); ERK; ERK-1; ERT 2; ERT2; Extracellular Signal Regulated Kinase 1; Extracellular signal related kinase 1; Extracellular signal-regulated kinase 1; HGNC6877; HS44KDAP; HUMKER1A; *** Stimulated MAP2 Kinase; ***-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP Kinase; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK; MAPK1; Mapk3; MGC20180; Microtubule Associated Protein 2 Kinase; Microtubule-associated protein 2 kinase; Mitogen Activated Protein Kinase 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 3; MK03_HUMAN; OTTHUMP00000174538; OTTHUMP00000174541; p44 ERK1; p44 MAPK; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM 3; PRKM3; Protein Kinase Mitogen Activated 3.  
   
产品类型 ***酸化抗体 
研究领域 神经生物学  信号转导  干细胞  激酶和***酸酶  Alzheimer's  
抗体来源 Mouse
克隆类型 Monoclonal
克 隆 号 9B6
交叉反应 Human, Mouse, Rat, Cow, Rabbit, Guinea Pig, Danio rerio,
产品应用 IHC-P=1:200-500 IHC-F=1:200-500 ICC=1:100-500 IF=1:200-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 43kDa
细胞定位 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human ERK1 around the phosphorylation site of Tyr222: 
亚    型 IgG
纯化方法 affinity purified by Protein G
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.

Function:
Serine/threonine kinase which acts as an essentialcomponent of the MAP kinase signal transduction pathway. MAPK1/ERK2and MAPK3/ERK1 are the 2 MAPKs which play an important role in theMAPK/ERK cascade. They participate also in a signaling cascadeinitiated by activated KIT and KITLG/SCF. Depending on the cellularcontext, the MAPK/ERK cascade mediates diverse biological functionssuch as cell growth, adhesion, survival and differentiation throughthe regulation of transcription, translation, cytoskeletalrearrangements. The MAPK/ERK cascade plays also a role ininitiation and regulation of meiosis, mitosis, and postmitoticfunctions in differentiated cells by phosphorylating a number oftranscription factors. About 160 substrates have already beendiscovered for ERKs. Many of these substrates are localized in thenucleus, and seem to participate in the regulation of transcriptionupon stimulation. However, other substrates are found in thecytosol as well as in other cellular organelles, and those areresponsible for processes such as translation, mitosis andapoptosis. Moreover, the MAPK/ERK cascade is also involved in theregulation of the endosomal dynamics, including lysosome processingand endosome cycling through the perinuclear recycling compartment(PNRC); as well as in the fragmentation of the Golgi apparatusduring mitosis. The substrates include transcription factors (suchas ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements(such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1),regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3,MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and avariety of other signaling-related molecules (like ARHGEF2, FRS2 orGRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2,RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2,RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases(such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates whichenable the propagation the MAPK/ERK signal to additional cytosolicand nuclear targets, thereby extending the specificity of thecascade.

Subunit:
Binds both upstream activators and downstream substratesin multimolecular complexes. Found in a complex with at least BRAF,HRAS1, MAP2K1/MEK1, MAPK3 and RGS14. Interacts with ADAM15, ARRB2,CANX, DAPK1 (via death domain), HSF4, IER3, MAP2K1/MEK1, MORG1,NISCH, PEA15, SGK1 and MKNK2 (By similarity). MKNK2 isoform 1binding prevents from dephosphorylation and inactivation. Interactswith TPR.

Subcellular Location:
Cytoplasm. Nucleus.Note=Autophosphorylation at Thr-207 promotes nuclear localization. PEA15-binding redirects the biological outcome ofMAPK3 kinase-signaling by sequestering MAPK3 into the cytoplasm.
Isoform 2: Nucleus.

Tissue Specificity:
Highest levels within the nervous system,expressed in different tissues, mostly in intestine, placenta andlung.

Post-translational modifications:
Phosphorylated upon FLT3 and KIT signaling. Ligand-activatedALK induces tyrosine phosphorylation.Dephosphorylated by PTPRJ at Tyr-205. Duallyphosphorylated on Thr-203 and Tyr-205, which activates the enzyme.

Similarity:
Belongs to the protein kinase superfamily. CMGCSer/Thr protein kinase family. MAP kinase subfamily. 
Contains 1 protein kinase domain.

SWISS:
P27361

Gene ID:
5595

Database links:

Entrez Gene: 5594 Human

Entrez Gene: 5595 Human

Entrez Gene: 116590 Rat

Entrez Gene: 50689 Rat

SwissProt: P27361 Human

SwissProt: P28482 Human

SwissProt: P21708 Rat

SwissProt: P63086 Rat

 

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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