Mouse Anti-phospho-CaMK2 beta/gamma/delta (Thr287) antibody
|英文名称||phospho-CaMK2 beta/gamma/delta (Thr287)|
|别 名||CaMK2 beta gamma delta (phospho Thr287); CaMK2 beta gamma delta (phospho T287); CaMK2 beta; CaMK2 gamma; CaMK2 delta; CAMK2G; Calcium/calmodulin dependent protein kinase (CaM kinase) II beta; Calcium/calmodulin dependent protein kinase II beta; Calcium/calmodulin dependent protein kinase IIB; Calcium/calmodulin dependent protein kinase type II beta chain; CAM 2; CaM kinase II beta chain; CaM kinase II beta subunit; CAM2; CAMK 2; CAMK 2B; CaMK II beta subunit; CaMK II subunit beta; CAMK2; CaMK2 beta; CAMK2B; CAMKB; CaMKII beta subunit; CaMKIIB; MGC29528; Proline rich calmodulin dependent protein kinase; CaM kinase II; Calcium/calmodulin-dependent protein; CaM kinase II gamma subunit; CaM-kinase II gamma chain; CaM kinase II delta subunit; CaM-kinase II delta chain; CAM2; CAMK; CAMK-II; CAMK2D; CAMK2G; CAMK2N2; CAMKD; CAMKG; KCC2B; kinase CaMK2-beta; KCC2B_HUMAN. CaMKIIβ/γ/δ (Phospho Thr287); CaMKIIβ/γ/δ (Phospho T287);|
|研究领域||肿瘤 免疫学 神经生物学 信号转导 激酶和***酸酶|
|克 隆 号||6G2|
|交叉反应||Human, Mouse, Rat,|
|产品应用||IHC-P=1:500-1000 IHC-F=1:500-1000 ICC=1:100-500 IF=1:500-1000 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||72kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthesised phosphopeptide derived from human CaMK2 beta/gamma/delta around the phosphorylation site of Ter286 [QE(p-T)VE]:|
|纯化方法||affinity purified by Protein G|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
The multifunctional CaMKII beta, or Ca2+/calmodulin-dependent protein kinase II, is a well known effector of calcium- and calmodulin- mediated functions. It is present in many tissues but is most abundant in the brain. CaMKII is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Autophosphorylation plays an important role in the regulation of the kinase activity. CaMKII is required for synaptic plasticity, as in Long Term Potentiation (LTP), a cellular model for learning and memory.
Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca2+/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca2+ transport and in fast-twitch muscle participates in the control of Ca2+ release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.
CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other. Interacts with SYNGAP1 and CAMK2N2 (By similarity). Interacts with MPDZ.
Cytoplasm › cytoskeleton
Cytoplasm › cytoskeleton › microtubule organizing center › centrosome
Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side
Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle.
Autophosphorylation of Thr-287 following activation by Ca2+/calmodulin. Phosphorylation of Thr-287 locks the kinase into an activated state.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
Entrez Gene: 525416 Cow
Entrez Gene: 816 Human
Entrez Gene: 12323 Mouse
Entrez Gene: 24245 Rat
Entrez Gene: 399284 Xenopus laevis
Omim: 607707 Human
SwissProt: Q3MHJ9 Cow
SwissProt: Q13554 Human
SwissProt: P28652 Mouse
SwissProt: P08413 Rat
Unigene: 351887 Human
Unigene: 439733 Mouse
Unigene: 9743 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.