3-磷酸甘油醛脱氢酶抗体上海沪震实业有限公司

性能参数

产品名称: 3-磷酸甘油醛脱氢酶抗体
英文名称: GAPDH
抗体货号: hz-2188R
产品规格: 50ul/100ul/200ul
级    别: , , 分析纯,
产品产地: 中国/上海
品牌商标: HZbscience
价    格: 580-1600元
抗原: IgG
抗原来源: Rabbit
抗体来源: Rabbit
适用物种: 见说明书
应用范围: WB=1:2000-10000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500
更新时间: 2018/4/18 14:48:00
详细资料:  实验方法技术资料
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使用范围:仅限科研使用,不能应用于临床
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产品详细描述

 Rabbit Anti-GAPDH  antibody

 

产品编号 hz-2188R
英文名称 GAPDH
中文名称 3-***酸甘油醛脱氢酶抗体
   
产品类型 内参抗体 
研究领域 免疫学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Rabbit, 
产品应用 WB=1:2000-10000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 37kDa
细胞定位 细胞核 细胞浆 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 GAPDH protein of rabbit: 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 38kDa subunits.

Function:
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Subunit:
Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with EIF1AD, USP25, PRKCI and WARS.

Subcellular Location:
Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions.

Post-translational modifications:
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated (Probable).
Sulfhydration at Cys-152 increases catalytic activity.

Similarity:
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

SWISS:
P04406

Gene ID:
100009074

Database links:

Entrez Gene: 493876 Cat

Entrez Gene: 374193 Chicken

Entrez Gene: 403755 Dog

Entrez Gene: 2597 Human

Entrez Gene: 100042025 Mouse

Entrez Gene: 14433 Mouse

Entrez Gene: 396823 Pig

Entrez Gene: 100009074 Rabbit

Entrez Gene: 24383 Rat

Entrez Gene: 685186 Rat

Entrez Gene: 380259 Xenopus laevis

Entrez Gene: 448356 Xenopus tropicalis

Entrez Gene: 317743 Zebrafish

Omim: 138400 Human

SwissProt: Cat

SwissProt: P00356 Chicken

SwissProt: Q28259 Dog

SwissProt: P70685 Guinea pig

SwissProt: P04406 Human

SwissProt: P16858 Mouse

SwissProt: P00355 Pig

SwissProt: P46406 Rabbit

SwissProt: P04797 Rat

SwissProt: P51469 Xenopus laevis

SwissProt: Q5XJ10 Zebrafish

Unigene: 544577 Human

Unigene: 592355 Human

Unigene: 598320 Human

Unigene: 304088 Mouse

Unigene: 309092 Mouse

Unigene: 317779 Mouse

Unigene: 343110 Mouse

Unigene: 392463 Mouse

Unigene: 392480 Mouse

Unigene: 414470 Mouse

Unigene: 458138 Mouse

Unigene: 458416 Mouse

Unigene: 475698 Mouse

Unigene: 129558 Rat

Unigene: 91450 Rat

Unigene: 995 Xenopus laevis

Unigene: 35640 Zebrafish



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

甘油醛-3-***酸脱氢酶(Glyceraldehyde 3 phosphate dehydrogenase,GAPDH)是糖酵解(glycolysis)过程中的关键酶。除了在胞质中作为糖酵解的酶以外,有证据表明哺乳动物细胞中的GAPDH参与了多种胞内生化过程,包括膜融合(membrane fusion)、微管成束(microtubule bundling)、***酸转移酶(phosphotransferase)激活、核内RNA出核、DNA复制与DNA修复。一些生理因素,诸如低氧(hypoxia)和尿糖(diabetes),可以增加GAPDH在特定细胞中的表达。GAPDH存在于几乎所有的组织中,以高水平持续表达。 GAPDH(甘油醛-3-***酸脱氢酶)是参与糖酵解的一种关键酶,由4个30-40kDa的亚基组成。
产品图片
Sample: 
U251(Human) Cell Lysate at 30 ug
Primary: Anti-GAPDH (bs-2188R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD
Sample: 
Hela Cell (Human) Lysate at 40 ug
MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti- GAPDH (bs-2188R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD
Sample: 
Liver(Rat) Lysate at 40 ug
Primary: Anti-GAPDH (bs-2188R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 35 kD
Sample: 
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-GAPDH (bs-2188R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 35 kD
Sample: 
Muscle (Rat) Lysate at 40 ug
Heart (Rat) Lysate at 40 ug
Primary: Anti- GAPDH (bs-2188R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD
Sample: Muscle (Mouse) Lysate at 40 ug
Primary: Anti- GAPDH (bs-2188R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD
Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti- GAPDH(bs-2188R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-GAPDH Polyclonal Antibody, Unconjugated(bs-2188R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-GAPDH Polyclonal Antibody, Unconjugated(bs-2188R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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