微管蛋白β tubulin/Tubulin β抗体上海沪震实业有限公司

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产品名称: 微管蛋白β tubulin/Tubulin β抗体
英文名称: Beta tubulin
抗体货号: hz-4511R
产品规格: 50ul/100ul/200ul
级    别: , , 分析纯,
产品产地: 中国/上海
品牌商标: HZbscience
价    格: 580-1600元
抗原: IgG
抗原来源: Rabbit
抗体来源: Rabbit
适用物种: 见说明书
应用范围: WB=1:2000-10000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500
更新时间: 2018/9/1 15:46:00
详细资料:  实验方法技术资料
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使用范围:仅限科研使用,不能应用于临床
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产品详细描述

 Rabbit Anti-Beta tubulin  antibody

 

产品编号 hz-4511R
英文名称 Beta tubulin
中文名称 微管蛋白β tubulin/Tubulin β抗体
别    名 Beta 4 tubulin; Tubulin-beta; Tubulin beta; Beta 5 tubulin; BetaTubulin; Beta-Tubulin; dJ40E16.7; TUBB; TUBB2; TUBB2A; TUBB5; tubulin beta 2A; Tubulin beta chain; Tubulin beta-5 chain; TUBB4A; TUBB4; Tubulin 5 beta; Tubulin beta-4 chain; TBB4A_HUMAN; Tubulin beta-4A chain.  
   
产品类型 内参抗体 
研究领域 免疫学  神经生物学  细胞骨架  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Rabbit, 
产品应用 WB=1:2000-10000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 55kDa
细胞定位 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human tubulin Beta:61-160/444 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) and therefore beta Tubulin should not be used as loading control for these tissues. 

Function:
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. 

Subunit:
Dimer of alpha and beta chains. May interact with RNABP10 (By similarity). Interacts with PIFO. Interacts with MX1 (By similarity). 

Subcellular Location:
Cytoplasm, cytoskeleton.

Tissue Specificity:
Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Post-translational modifications:
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable). 

Similarity:
Belongs to the tubulin family.

SWISS:
P07437

Gene ID:
203068

Database links:

Entrez Gene: 203068 Human

Omim: 191130 Human

SwissProt: P07437 Human

SwissProt: P99024 Mouse

SwissProt: P69897 Rat

 



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

结构蛋白(Structural Proteins)
tubulin是一种大量存在于哺乳动物脑组织中的微管亚基蛋白,在结构上是由两个极为相近的α和β亚基组成的二聚体、多聚体形成微管细丝,是微管的主要成分。
微管蛋白是球形分子, 有两种类型:α微管蛋白(α-tubulin)货号:bs-0195R和β微管蛋白(β-tubulin), 这两种微管蛋白具有相似的三维结构, 能够紧密地结合成二聚体, 作为微管组装的亚基。 α亚基由450个氨基酸组成, β亚基是由455个氨基酸组成, 这两种亚基有35~40%的氨基酸序列同源, 表明编码它们的基因可能是由同一原始祖先演变而来.
产品图片
Sample: 
Cerebrum (Rat) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- Beta tubulin (bs-4511R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample: 
Brain (mouse) Lysate at 40 ug
Heart (mouse) Lysate at 40 ug
Primary: Anti- beta tubulin(bs-4511R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample: 
Cerebrum (Mouse)Cell Lysate at 40 ug
Primary: Anti-Beta tubulin (bs-4511R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55kD
Sample: Spleen (Mouse) Lysate at 40 ug
Primary: Anti- Beta tubulin (bs-4511R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample: 
Heart (Mouse) Lysate at 40 ug
MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti-Beta tubulin (bs-4511R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample: 
Brain (Rat) Lysate at 40 ug
Heart (Rat) Lysate at 40 ug
Primary: Anti-Beta tubulin (bs-4511R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 55 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin ) Polyclonal Antibody, Unconjugated (bs-4511R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-Tubulin beta Polyclonal Antibody, Unconjugated(bs-4511R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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