乙酰化组蛋白H4抗体上海沪震实业有限公司

性能参数

产品名称: 乙酰化组蛋白H4抗体
英文名称: Histone H4 (Acetyl K5)
抗体货号: hz-4706R
产品规格: 100ul
级    别: , , 分析纯,
产品产地: 中国/上海
品牌商标: HZbscience
价    格: 1580元
抗原: IgG
抗原来源: Rabbit
抗体来源: Rabbit
适用物种: 见说明书
应用范围: WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:50-200
更新时间: 2018/4/18 16:30:00
详细资料:  实验方法技术资料
浏览人数:12
诚信指数:730点
了解更多:进入公司展台
使用范围:仅限科研使用,不能应用于临床
点此求购
在线QQ: 我是1594652376,请问我有什么可以帮到您呢?
生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

供应商联系卡

上海沪震实业有限公司
地址:
上海市杨浦区密云路1018号复旦科技园808室
邮编:
200612
电话:
021-60345367
传真:
021-31320307
联系人:
陈先生
所在区域:
上海·中国-上海
邮件:
公司展台:
在线QQ:
我是1594652376,请问我有什么可以帮到您呢?
扫一扫,关注我们

产品详细描述

 Rabbit Anti-Histone H4 (Acetyl K5) antibody 

 

产品编号 hz-4706R
英文名称 Histone H4 (Acetyl K5)
中文名称 乙酰化组蛋白H4抗体
别    名 Histone H4 (Acetyl K5); Histone H4 (Acetyl Lys5); Acetyl-Histone H4(K5); Acetyl-Histone H4 (Lys5); H4F2; H4FN; HIST1H4; HIST2H4; HISTH4H4; methyl histone H4; histone H4; H4_HUMAN; Osteogenic growth peptide; OGP.  
   
产品类型 乙酰化抗体 
研究领域 细胞生物  免疫学  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:50-200 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 11kDa
细胞定位 细胞核 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated Synthesised acetylpeptide derived from human Histone H4 around the acetylation site of K5:RG(Ac-K)GG 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008].

Function:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Subunit:
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

Subcellular Location:
Nucleus. Chromosome.

Post-translational modifications:
Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin. 
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation. 
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. 
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing. 
Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. 
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). 
Sumoylated, which is associated with transcriptional repression. 
Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.

Similarity:
Belongs to the histone H4 family.

SWISS:
P02304

Gene ID:
121504

Database links:

Entrez Gene: 121504 Human

Entrez Gene: 554313 Human

Entrez Gene: 8294 Human

Entrez Gene: 8359 Human

Entrez Gene: 8360 Human

Entrez Gene: 8361 Human

Entrez Gene: 8362 Human

Entrez Gene: 8363 Human

Entrez Gene: 8364 Human

Entrez Gene: 8365 Human

Entrez Gene: 8366 Human

Entrez Gene: 8367 Human

Entrez Gene: 8368 Human

Entrez Gene: 8370 Human

Entrez Gene: 100041230 Mouse

Entrez Gene: 100862646 Mouse

Entrez Gene: 319155 Mouse

Entrez Gene: 319156 Mouse

Entrez Gene: 319157 Mouse

Entrez Gene: 319158 Mouse

Entrez Gene: 319159 Mouse

Entrez Gene: 319160 Mouse

Entrez Gene: 319161 Mouse

Entrez Gene: 320332 Mouse

Entrez Gene: 326619 Mouse

Entrez Gene: 326620 Mouse

Entrez Gene: 69386 Mouse

Entrez Gene: 97122 Mouse

GenBank: NM_003548 Human

Omim: 142750 Human

SwissProt: P84040 Fruit fly (Drosophila melanogaster)

SwissProt: P02304 Human

SwissProt: P62805 Human

SwissProt: P02304 Mouse

SwissProt: P62806 Mouse

SwissProt: P02304 Rat

SwissProt: P09322 Schizosaccharomyces pombe

Unigene: 21500 Fruit fly (Drosophila melanogaster)

Unigene: 29514 Fruit fly (Drosophila melanogaster)

Unigene: 29527 Fruit fly (Drosophila melanogaster)

Unigene: 30219 Fruit fly (Drosophila melanogaster)

Unigene: 30220 Fruit fly (Drosophila melanogaster)

Unigene: 30221 Fruit fly (Drosophila melanogaster)

Unigene: 30223 Fruit fly (Drosophila melanogaster)

Unigene: 30868 Fruit fly (Drosophila melanogaster)

Unigene: 30869 Fruit fly (Drosophila melanogaster)

Unigene: 30871 Fruit fly (Drosophila melanogaster)

Unigene: 30872 Fruit fly (Drosophila melanogaster)

Unigene: 30873 Fruit fly (Drosophila melanogaster)

Unigene: 30876 Fruit fly (Drosophila melanogaster)

Unigene: 33873 Fruit fly (Drosophila melanogaster)

Unigene: 5747 Fruit fly (Drosophila melanogaster)

Unigene: 143080 Human

Unigene: 247816 Human

Unigene: 248172 Human

Unigene: 248178 Human

Unigene: 248179 Human

Unigene: 278483 Human

Unigene: 352191 Human

Unigene: 46423 Human

Unigene: 528055 Human

Unigene: 533295 Human

Unigene: 55468 Human

Unigene: 591790 Human

Unigene: 655235 Human

Unigene: 662174 Human

Unigene: 706635 Human

Unigene: 742244 Human

Unigene: 14775 Mouse

Unigene: 158272 Mouse

Unigene: 227295 Mouse

Unigene: 228709 Mouse

Unigene: 246720 Mouse

Unigene: 255646 Mouse

Unigene: 260530 Mouse

Unigene: 261642 Mouse

Unigene: 261662 Mouse

Unigene: 261664 Mouse

Unigene: 377875 Mouse

Unigene: 442307 Mouse

Unigene: 486099 Mouse

Unigene: 489077 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
 
产品图片
Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (H4 (Acetyl K5)) Polyclonal Antibody, Unconjugated (bs-4706R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

查看乙酰化组蛋白H4抗体产品的用户还对以下产品感兴趣

暂无

ADVERTISEMENT

找不到您所需的产品,发布求购试试?

标题:*
描述:
姓名:*
Email:*
单位:*
电话:*
地址:*
邮编:
资料: 需要 不需要
报价: 需要 不需要