FITC标记的核受体0相关蛋白B家族2抗体
产品名称： FITC标记的核受体0相关蛋白B家族2抗体
英文名称： Anti-NR0B2/FITC
产品编号： HZ-
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
上海沪震实业有限公司
- 联系人 : 鲍丽雯
- 地址 : 上海市闵行区闵北路88弄1-30号第22幢AQ136室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 139****0749
- 传真 : 021-60345367
- 邮箱 : www.shzbio.net
Rabbit Anti-NR0B2/FITC Conjugated antibody
FITC标记的核受体0相关蛋白B家族2抗体
| 产品编号 | bs-4311R-FITC |
| 英文名称 | Anti-NR0B2/FITC |
| 中文名称 | FITC标记的核受体0相关蛋白B家族2抗体 |
| 别 名 | NR0B2; NR0B2_HUMAN; Nr0b2a; Nuclear receptor subfamily 0 group B member 2; Nuclear receptor subfamily 0, group B, member 2a; Orphan nuclear receptor SHP; SHP; SHP-1; Shp1; Small heterodimer partner. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 细胞生物 染色质和核信号 信号转导 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 28kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NR0B2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: SHP is an orphan nuclear receptor containing the dimerization and ligand-binding domains found in other nuclear receptors, but lacking the conserved DNA binding domain. SHP is specifically expressed in liver and other tissues, including fetal liver and adrenal gland, as well as adult spleen and small intestine. In addition, SHP is highy expressed in the murine macrophage cell line RAW 264.7 but suppressed by oxLDL and 13-HODE, which is a ligand for PPARg. SHP interacts with nuclear receptors, including thyroid receptor, retinoic acid receptors (RAR and RXR) and estrogen receptors (ERa and ERb). SHP functions as a negative regulator of these receptors by at least three mechanisms: inhibition of DNA binding via dimerization, direct antagonism of coactivator function through competition and possibly transrepression via recruitment of putative corepressors. In oxLDL-treated, resting macrophage cells, SHP acts as a transcription coactivator of NFkB, suggesting that SHP is a modulatory component in the regulation of the transcriptional activities of NFkB. Lastly, negative feedback regulation of a hepatic bile acid transporter, NTCP, is controlled by bile acid-activated FXR via induction of SHP to protect the hepatocyte from bile acid-mediated damage in cholestatic conditions. Function: Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Subunit: Interacts with RARA, RXRA, THRB, NR5A1, NR5A2, NR1I3, PPARA, PPARG and EID1. May also interact with HNF4A (By similarity). Heterodimer; efficient DNA binding requires dimerization with another bHLH protein. Interacts (via N-terminus) with NEUROD1 (via N-terminus an C-terminus). Interacts with ID2. Subcellular Location: Nucleus. Cytoplasm. Note=Colocalizes with NEUROD1 in the nucleus. Tissue Specificity: Liver. Low levels of expression were detected in heart and pancreas. DISEASE: Defects in NR0B2 may be associated with obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Similarity: Belongs to the nuclear hormone receptor family. NR0 subfamily. Database links: Entrez Gene: 8431 Human Omim: 604630 Human SwissProt: Q15466 Human Unigene: 427055 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
SHP是一种孤儿核受体，含有其他核受体中的二聚和配体结合结构域，但缺乏保守的DNA结合结构域。在肝脏和其他组织中，尤其是胎儿肝脏和肾上腺，以及脾脏和小肠，SHP特异表达。此外，SHP在小鼠巨噬细胞系RAW 264.7中高表达，但被oxLDL和13-HODE抑制，这是PPARG的配体。SHP与核受体相互作用，包括甲状腺受体、视黄酸受体（RAR和RXR）和雌激素受体（ERA和厄尔布）。SHP通过至少三种机制作为这些受体的负调节器：通过二聚化抑制DNA结合，通过竞争竞争直接激活共激活因子功能，并通过招募假定的辅阻遏物可能进行阻遏作用。在oxLDL处理的静息巨噬细胞中，SHP作为NFKB的转录辅激活子，提示SHP是调节NFKB转录活性的调节成分。最后，通过胆汁酸激活的FXR控制肝胆汁酸转运蛋白NTCP的负反馈调节，以诱导肝细胞免受胆汁淤积条件下的胆汁酸介导的损伤。