Rabbit Anti-Frizzled 2/FITC Conjugated antibody
|别 名||Frizzled homolog 2; Frizzled-2; Frizzled2; Fz 2; Fz-2; Fz2; FZD 2; FZD2; FZD2_HUMAN; FzE 2; FzE2; hFz 2; hFz2.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||肿瘤 细胞生物 信号转导 干细胞 转录调节因子 G蛋白偶联受体 细胞表面分子 G蛋白信号|
|交叉反应||Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||60kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthetic peptide derived from human Frizzled 2|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
The frizzled gene, originally identified in Drosophila melanogaster, is involved in the development of tissue polarity. The mammalian homolog of frizzled as well as several secreted mammalian frizzled-related proteins (FRPs) have been described. The frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a carboxy terminal Ser/Thr-xxx-Val motif. They function as receptors for Wnt and are generally coupled to G proteins. Expression of frizzled-2 can be observed in the fetal kidney and lung and in the adult ovary and colon. The Wnt/cGMP/Ca2+ pathway is mediated by frizzled-2 . It binds Wnt proteins and signals by activating the release of stored calcium. Expression of frizzled-2 is regulated by Angiotensin II. Activated frizzled-2 suppresses the activity of protein kinase G, and activates NFAT-dependent transcription, the phosphatidylinositol pathway and calcium sensitive enzymes.
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Widely expressed. In the adult, mainly found in heart, placenta, skeletal muscle, lung, kidney, pancreas, prostate, testis, ovary and colon. In the fetus, expressed in brain, lung and kidney. Low levels in fetal liver.
Ubiquitinated by ZNRF3, leading to its degradation by the proteasome (By similarity).
Belongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain.
Entrez Gene: 2535 Human
Entrez Gene: 57265 Mouse
Entrez Gene: 64512 Rat
Omim: 600667 Human
SwissProt: Q14332 Human
SwissProt: Q9JIP6 Mouse
SwissProt: Q08464 Rat
Unigene: 142912 Human
Unigene: 597264 Human
Unigene: 36416 Mouse
Unigene: 92324 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications
最初在果蝇中发现的卷曲基因参与了组织极性的发育。本文描述了卷曲的哺乳动物同源物以及几种分泌的哺乳动物卷曲相关蛋白（FRPS）。卷曲蛋白含有七个跨膜结构域，胞外区富含半胱氨酸结构域和羧基末端S/THR XXX VAL基序。它们作为Wnt的受体，通常与G蛋白结合。在胎儿肾脏和肺和***卵巢和结肠中可观察到FRZZED-2的表达。Wnt/cGMP/Ca2+通路是由FruZel-2介导的。它通过激活储存的钙释放来结合Wnt蛋白和信号。FRZZED-2的表达受到Angiotensin II的调控。激活的FruZED-2抑制蛋白激酶G的活性，并激活NFAT依赖的转录，***脂酰肌醇途径和钙敏感酶。