Rabbit Anti-TORC2/FITC Conjugated antibody
|别 名||CREB regulated transcription coactivator 2; CRTC2; RP11-422P24.6; TORC-2; Transducer of CREB protein 2; Transducer of regulated cAMP response element-binding protein; Transducer of regulated cAMP response element-binding protein (CREB) 2; Transducer of regulated cAMP response element-binding protein 2; Transducer of regulated CREB protein 2; CRTC2_HUMAN.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||肿瘤 免疫学 信号转导 细胞凋亡 转录调节因子 激酶和***酸酶|
|交叉反应||Human, Mouse, Rat, Dog, Pig, Cow,|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||76kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthetic peptide derived from human CREB regulated transcription coactivator 2|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
This gene encodes a member of the transducers of regulated cAMP response element-binding protein activity family of transcription coactivators. These proteins promote the transcription of genes targeted by the cAMP response element-binding protein, and therefore play an important role in many cellular processes. Under basal conditions the encoded protein is phosphorylated by AMP-activated protein kinase or the salt-inducible kinases and is sequestered in the cytoplasm. Upon activation by elevated cAMP or calcium, the encoded protein translocates to the nucleus and increases target gene expression. Single nucleotide polymorphisms in this gene may increase the risk of type 2 diabetes. A pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2010].
Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
Binds, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1. 'Arg-314' in the bZIP domain of CREB1 is essential for this interaction. Interaction, via its C-terminal, with TAF4, enhances recruitment of TAF4 to CREB1. Interacts with PPP3CA/calcineurin alpha, SIK2 and 14-3-3 proteins, YWHAB and YWHAG. Interaction with the human T-cell leukemia virus type 1 (HTLV-1) Tax protein is essential for optimal transcription activation by Tax. Interaction with RFWD2/COP1 mediates nuclear export and degradation of CRTC2.
Cytoplasm. Nucleus. Note=Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein. In response to cAMP levels and glucagon, relocated to the nucleus.
Most abundantly expressed in the thymus. Present in both B and T-lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas.
Phosphorylation/dephosphorylation states of Ser-171 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation, is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs (SIK1 and SIK2) by LKB1. Both insulin and AMPK increase this phosphorylation of CRTC2 while glucagon suppresses it. Phosphorylation at Ser-274 by MARK2 is induced under low glucose conditions and dephosphorylated in response to glucose influx. Phosphorylation at Ser-274 promotes interaction with 14-3-3 proteins and translocation to the cytoplasm.
Belongs to the TORC family.
Entrez Gene: 200186 Human
Entrez Gene: 74343 Mouse
Entrez Gene: 310615 Rat
Omim: 608972 Human
SwissProt: Q53ET0 Human
SwissProt: Q3U182 Mouse
SwissProt: Q3LRZ1 Rat
Unigene: 406392 Human
Unigene: 35627 Mouse
Unigene: 13599 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
该基因编码调控cAMP应答元件结合蛋白活性家族的转录辅激活子的成员。这些蛋白促进cAMP应答元件结合蛋白靶向基因的转录，因此在许多细胞过程中起重要作用。在碱性条件下，编码的蛋白通过AMP活化蛋白激酶或盐诱导激酶***酸化，并被隔离在细胞质中。经升高的cAMP或钙激活后，编码的蛋白质转位到细胞核并增加靶基因的表达。该基因中的单核苷酸多态性可能增加2型糖尿病的风险。该基因的假基因位于5号染色体的长臂上。[ RefSeq，DEC 2010 ]提供。