Rabbit Anti-Phospho-RASGRF1 (Ser916)/FITC Conjugated antibody
|别 名||RASGRF1(phospho S916)CDC25; CDC25L; GNRP; GRF1; GRF55; Guanine nucleotide exchange factor; Guanine nucleotide releasing factor 55 kD; Guanine nucleotide releasing protein; HGRF55; PP13187; Ras protein specific guanine nucleotide releasing factor 1; Ras specific guanine nucleotide releasing factor; Ras specific guanine nucleotide releasing factor CDC25 homolog; Ras specific nucleotide exchange factor CDC25.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||肿瘤 免疫学 信号转导 转录调节因子|
|交叉反应||Human, Mouse, Rat,|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||55kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated Synthesised phosphopeptide derived from human RasGRF1 around the phosphorylation site of Ser916|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Mar 2009]
Promotes the exchange of Ras-bound GDP by GTP.
Homooligomer and heterooligomer with RASGRF2. Interacts with USP8, thereby regulating its stability.
Phosphorylated by PLK2, leading to ubiquitination and degradation by the proteasome.
Ubiquitinated and degraded following phosphorylation by PLK2 By similarity).
Phosphorylated by SRC and LCK. Phosphorylation by LCK increases its capacity to stimulate the GDP/GTP exchange on Ras, whereas its phosphorylation by SRC seems not to have an effect on stimulation activity.
Contains 1 DH (DBL-homology) domain.
Contains 1 IQ domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 2 PH domains.
Contains 1 Ras-GEF domain.
Entrez Gene: 5923 Human
Entrez Gene: 192213 Rat
Omim: 606600 Human
SwissProt: Q13972 Human
SwissProt: P28818 Rat
Unigene: 459035 Human
Unigene: 135776 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications
由该基因编码的蛋白质是类似于酿酒酵母CDC25基因产物的鸟嘌呤核苷酸交换因子（GEF）。功能分析表明，这种蛋白质刺激从Ras蛋白的GDP的解离。小鼠相似基因的研究表明，该蛋白在脑内的RAS GEF活性可被Ca2+内流、毒蕈碱受体和G蛋白β-γ亚单位激活。小鼠研究还表明，由该蛋白介导的RAS GEF信号通路可能对长期记忆很重要。另外，报道了编码不同亚型的剪接转录变体。〔RefSeq，2009〕