Rabbit Anti-Phospho-Raptor (Ser792)/FITC Conjugated antibody
|别 名||ARaptor (phospho S792); Raptor (phospho Ser792); p-Raptor (Ser792); p150 target of rapamycin (TOR) scaffold protein containing WD repeats; P150 target of rapamycin (TOR)-scaffold protein; Regulatory Associated Protein of mTOR; KIAA1303; RPTOR_HUMAN; Regulatory-associated protein of mTOR; p150 target of rapamycin (TOR)-scaffold protein.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||肿瘤 免疫学 信号转导 转录调节因子|
|交叉反应||Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep, Guinea Pig, Danio rerio|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||140kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated Synthesised phosphopeptide derived from human Raptor around the phosphorylation site of Ser792|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
mTOR controls cell growth, in part by regulating p70 S6 kinase alpha (p70alpha) and eukaryotic initiation factor 4E binding protein 1 (4EBP1). Raptor is a 150 kDa mTOR binding protein that also binds 4EBP1 and p70alpha. The binding of Raptor to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70alpha. Rapamycin or amino acid withdrawal increases, whereas insulin strongly inhibits, the recovery of 4EBP1 and raptor on 7-methyl-GTP Sepharose. Partial inhibition of raptor expression by RNA interference (RNAi) reduces mTOR-catalyzed 4EBP1 phosphorylation in vitro. RNAi of C. elegans raptor yields an array of phenotypes that closely resemble those produced by inactivation of Ce-TOR. Thus, raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo.
Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.
Interacts with MTOR. Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR and AKT1S1. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Binds directly to 4EBP1 and RPS6KB1 independently of its association with MTOR. Binds preferentially to poorly or non-phosphorylated forms of EIF4EBP1, and this binding is critical to the ability of MTOR to catalyze phosphorylation. Forms a complex with MTOR under both leucine-rich and -poor conditions. Interacts with ULK1 in a nutrient-dependent manner; the interaction is reduced during starvation. Interacts (when phosphorylated by AMPK) with 14-3-3 protein, leading to inhibit its activity.
Cytoplasm. Lysosome. Note=Targeting to lysosomes depends on amino acid availability.
Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta.
In response to nutrient limitation, phosphorylated by AMPK; phosphorylation promotes interaction with 14-3-3 proteins, leading to negative regulation of the mTORC1 complex. In response to growth factors, phosphorylated at Ser-719, Ser-721 and Ser-722 by RPS6KA1; phosphorylation positively stimulates mTORC1 activity.
Belongs to the WD repeat RAPTOR family.
Contains 7 WD repeats.
Entrez Gene: 57521 Human
Entrez Gene: 74370 Mouse
Entrez Gene: 287871 Rat
Omim: 607130 Human
SwissProt: Q8N122 Human
SwissProt: Q8K4Q0 Mouse
Unigene: 133044 Human
Unigene: 209933 Mouse
Unigene: 98539 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
mTOR通过调节p70S6激酶α（p70alpha）和真核起始因子4E结合蛋白1（4EBP1）来控制细胞生长。猛禽是一个150 kDa mTOR结合蛋白，也结合4EBP1和P70AlpA。猛禽与mTOR的结合是体外mTOR催化4EBP1***酸化所必需的，它强烈地增强了pTORα的mTOR激酶活性。雷帕霉素或氨基酸撤出增加，而胰岛素强烈抑制，4EBP1和RAPTOR在7-甲基-GTP琼脂糖上的恢复。RNA干扰（RNAi）部分抑制RAPTOR表达降低了mTOR催化的4EBP1***酸化。R.N.线虫的RNAi产生一组与CE-Tor失活相似的表型。因此，猛禽是mTOR催化的4EBP1***酸化的重要支架，并介导Tor在体内的作用。