FITC标记的染色体浓缩调控蛋白1抗体
产品名称: FITC标记的染色体浓缩调控蛋白1抗体
英文名称: Anti-RCC1/FITC
产品编号: HZ-3545R-FITC
产品价格: null
产品产地: 中国/上海
品牌商标: HZbscience
更新时间: 2023-08-17T10:24:20
使用范围: IF=1:50-200
上海沪震实业有限公司
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Rabbit Anti-RCC1/FITC Conjugated antibody
FITC标记的染色体浓缩调控蛋白1抗体
文名称 | Anti-RCC1/FITC |
中文名称 | FITC标记的染色体浓缩调控蛋白1抗体 |
别 名 | Cell cycle regulatory protein; CHC 1; CHC1; Chromosome condensation 1; Chromosome condensation protein 1; Guanine nucleotide releasing protein; HERC2; Ran GEF; RanGEF; RCC 1; RCC1 I; Regulator of chromosome condensation 1; Regulator of chromosome condensation; SHEP1; RCC1_HUMAN. |
规格价格 | 100ul/2980元 购买 大包装/询价 |
说 明 书 | 100ul |
研究领域 | 细胞生物 染色质和核信号 信号转导 转录调节因子 激酶和磷酸酶 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, Mouse, Rat, Pig, Cow, |
产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 46kDa |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human RCC1 (2-50aa) |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
产品介绍 | background: Ran GTPase plays important roles in nucleocytoplasmic transport in interphase and in both spindle formation and nuclear envelope (NE) assembly during mitosis. The latter functions rely on the presence of high local concentrations of GTP bound Ran near mitotic chromatin. RanGTP localization has been proposed to result from the association of Ran's GDP/GTP exchange factor, RCC1, with chromatin , but Ran is shown here to bind directly to chromatin in two modes, either dependent or independent of RCC1, and, where bound, to increase the affinity of chromatin for NE membranes. Function: Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly. Subunit: Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation. Subcellular Location: Nucleus. Cytoplasm. Note=Becomes dispersed throughout the cytoplasm during mitosis. Post-translational modifications: N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran. Similarity: Contains 7 RCC1 repeats. Database links: Entrez Gene: 1104 Human Entrez Gene: 100088 Mouse Entrez Gene: 682908 Rat Omim: 179710 Human SwissProt: P18754 Human SwissProt: Q8VE37 Mouse Unigene: 469723 Human Unigene: 255045 Mouse Unigene: 217123 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
Ran GTPase在有丝分裂过程中,在纺锤体形成和核膜(NE)组装中,在核质转运中起着重要的作用。后者的功能依赖于高局部浓度的GTP结合RAN附近有丝分裂染色质的存在。RANTP定位已被提出是由于RAN的GDP/GTP交换因子RC1与染色质的关联,但RAN在此示出在两种模式中直接与染色质结合,依赖于或独立于Rcc1,并且在结合的情况下增加染色质对NE-MEM的亲和性。膜。