FITC标记的磷酸化3磷酸肌醇依赖性蛋白激酶1
产品名称: FITC标记的磷酸化3磷酸肌醇依赖性蛋白激酶1
英文名称: Anti-Phospho-PDK1(Ser241)/FITC
产品编号: HZ-3327R-FITC
产品价格: null
产品产地: 中国/上海
品牌商标: HZbscience
更新时间: 2023-08-17T10:24:20
使用范围: IF=1:50-200
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Rabbit Anti-Phospho-PDK1(Ser241)/FITC Conjugated antibody
FITC标记的磷酸化3磷酸肌醇依赖性蛋白激酶1
英文名称 | Anti-Phospho-PDK1(Ser241)/FITC |
中文名称 | FITC标记的磷酸化3磷酸肌醇依赖性蛋白激酶1 |
别 名 | PDK1(Phospho-Ser241); PDPK1 (phospho S241); PDPK1(phospho-S241); 3 phosphoinositide dependent protein kinase 1; hPDK 1; hPDK1; MGC20087 MGC35290; PDK 1; PDPK 1; PDPK1; PkB kinase; PkB kinase like gene 1; PkB like 1; PkB like; PRO0461; PDPK1_HUMAN. |
规格价格 | 100ul/2980元 购买 大包装/询价 |
说 明 书 | 100ul |
产品类型 | 磷酸化抗体 |
研究领域 | 肿瘤 免疫学 神经生物学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, Mouse, Rat, Chicken, Pig, Cow, Rabbit, |
产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 61kDa |
细胞定位 | 细胞膜 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human PDPK1 around the phosphorylation site of Ser241 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
产品介绍 | background: PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation it is a potentially important target for metabolic drug research. There are three named isoforms. Function: Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive. Subunit: Interacts with NPRL2. Subcellular Location: Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane. Tissue Specificity: Appears to be expressed ubiquitously. Post-translational modifications: Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily. Contains 1 PH domain. Contains 1 protein kinase domain. Database links: Entrez Gene: 5170 Human Entrez Gene: 18607 Mouse Entrez Gene: 81745 Rat Omim: 605213 Human SwissProt: O15530 Human SwissProt: Q9Z2A0 Mouse SwissProt: O55173 Rat Unigene: 459691 Human Unigene: 10504 Mouse Unigene: 10905 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PDK1是蛋白激酶B(PKB)的上游激酶,通过与3,4,5三磷酸磷脂酰肌醇作用激活相邻的PKB分子 |
PDK1(3磷酸肌醇依赖性蛋白激酶1)磷酸化AGC激酶。PDK1通过激活环中的关键苏氨酸残基的磷酸化激活常规PKC和PKCζ。PDK1还在磷脂酰肌醇-3,4-,5-三磷酸存在下在苏氨酸308上磷酸化Protein Kinase B(PKB)。活性AKT失活糖原合成酶激酶3(GSK3),最终导致糖原合酶的去磷酸化和活化和糖原合成的刺激。由于PDK在胰岛素诱导的糖原合成和PKC活化中发挥的作用,它是代谢药物研究的潜在重要靶点。有三个命名的异构体。