Rabbit Anti-Phospho-PAK1(Ser199 + Ser204) + PAK2 (Ser192 + Ser197)/FITC Conjugated antibody
|英文名称||Anti-Phospho-PAK1(Ser199 + Ser204) + PAK2 (Ser192 + Ser197)/FITC|
|别 名||PAK1 + PAK2 (phospho S199 + S204); PAK2 (phospho S192 + S197); PAKalpha; serine/threonine-protein kinase PAK 1; p21 activated kinase 1; PAK alpha; Paka; PAKalpha; Protein kinase MUK2; STE20 homolog (yeast); Alpha PAK; p21/Cdc42/Rac1 activated kinase 1; CB422; EC 22.214.171.124; Gamma PAK; hPAK65; Kinase; p21 (CDKN1A)-activated kinase 2a; p21 activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 2; p21 protein Cdc42 Rac activated kinase 2; p21-activated kinase, 65-KD; p21-activated protein kinase I; p21CDKN1A activated kinase 2; p58; p65PAK; PAK 2; PAK65; PAKgamma; S6 H4 kinase; Serine threonine protein kinase PAK 2; Serine/threonine-protein kinase PAK 3; p21-activated kinase 3; PAK-3; Beta-PAK; PAK1_HUMAN; PAK2_HUMAN.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||肿瘤 免疫学 信号转导 细胞凋亡 转录调节因子 激酶和***酸酶|
|交叉反应||Human, Mouse, Rat, Rabbit,|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||60kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthesised phosphopeptide derived from human PAK1 around the phosphorylation site of Ser199/204|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
In mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death. P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), catalyzed by Cdc42, is required for activation of PAK.
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Phosphorylates MYL9/MLC2. Phosphorylates RAF1 at 'Ser-338' and 'Ser-339' resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2.
Homodimer in its autoinhibited state. Active as monomer. Interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. Binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1. Interacts with ARHGEF7. Also interacts with CRIPAK. Interacts with NISCH. Interacts with DVL1; mediates the formation of a DVL1, MUSK and PAK1 ternary complex involved in AChR clustering. Probably found in a ternary complex composed of DSCAM, PAK1 and RAC1. Interacts with DSCAM (via cytoplasmic domain); the interaction is direct and enhanced in presence of RAC1. Interacts with SCRIB. Interacts with PDPK1. Interacts (via kinase domain) with RAF1. Interaction with NCK1 and NCK2.
Cytoplasm. Cell junction, focal adhesion. Note=Recruited to focal adhesions upon activation.
Autophosphorylated when activated by CDC42/p21 and RAC1. Phosphorylation at Thr-423 by PDPK1 results in its activation.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
Entrez Gene: 5058 Human
Entrez Gene: 18479 Mouse
Entrez Gene: 29431 Rat
Omim: 602590 Human
SwissProt: Q13153 Human
SwissProt: O88643 Mouse
SwissProt: P35465 Rat
Unigene: 435714 Human
Unigene: 260227 Mouse
Unigene: 9149 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
在哺乳动物中，有几种被鉴定的p21活化蛋白激酶或PAKs的亚型：PAK1和PAK3主要是脑特异性的，而PAK2是普遍表达的。编码PAK3的基因突变与X连锁智力低下有关，PAK3是海马突触形成和可塑性的关键调节因子。PAK3在细胞形态、运动和细胞死亡的调控中起着关键作用。P21活化激酶（PAK）实际上是一个丝氨酸/苏氨酸蛋白激酶家族，其成员是由小分子量GTPs激活的。三种最常见的异构体分别是PAK 1、PAK 2和PAK 3（也称为α-PAK、γ-PAK和β-PAK）。这些激酶包含许多调控元件，触发不同的信号过程，如由激活的GTPase启动的，与Src同源性3（SH3）结构域的相互作用，和半胱氨酸天冬氨酸蛋白酶介导的蛋白水解裂解。由Cdc42催化的丝氨酸141（丝氨酸144对PAK 1和丝氨酸139 PAK 3的***酸化）是激活PAK所必需的。